| Regulation of mouse intestinal L cell progenitors proliferation by the glucagon family of peptides. | |
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MedLine Citation:
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PMID: 22569789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glucagon like peptide-1 (GLP-1) and GLP-2 are hormones secreted by intestinal L cells that stimulate glucose-dependent insulin secretion and regulate intestinal growth, respectively. Mice with deletion of the glucagon receptor (Gcgr) have high levels of circulating GLP-1 and GLP-2. We sought to determine whether the increased level of the glucagon-like peptides is due to L cell hyperplasia. We found, first, that high levels of the glucagon-like peptides increase L cell number but does not affect the number of other intestinal epithelial cell types. Second, a large proportion of ileal L cells of Gcgr(-/-) mice coexpressed glucose-dependent insulinotropic peptide (GIP). Cells coexpressing GIP and GLP-1 are termed LK cells. Third, the augmentation in L cell number was due to a higher rate of proliferation of L cell progenitors rather than to the entrance of mature L cells into the cell cycle. Fourth, a high concentration of the glucagon-like peptides in the circulation augmented the mRNA levels of transcription factors expressed by late but not early enteroendocrine progenitors. Fifth, the administration of exendin 9-39, a GLP-1 receptor antagonist, resulted in a decrease in the rate of L cell precursor proliferation. Finally, we determined that L cells do not express the GLP-1 receptor, suggesting that the effect of GLP-1 is mediated by paracrine and/or neuronal signals. Our results suggest that GLP-1 plays an important role in the regulation of L cell number. |
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Authors:
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Marine Grigoryan; Mamdouh H Kedees; Maureen J Charron; Yelena Guz; Gladys Teitelman |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-05-08 |
Journal Detail:
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Title: Endocrinology Volume: 153 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-06-25 Completed Date: 2012-09-20 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 3076-88 Citation Subset: AIM; IM |
Affiliation:
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Department of Cell Biology, State University of New York-Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Cell Cycle Cell Proliferation Crosses, Genetic Enteroendocrine Cells / cytology*, metabolism Gene Expression Regulation* Glucagon / metabolism* Goblet Cells / cytology Intestines / cytology* Male Mice Mice, Knockout Mice, Transgenic Neurons / metabolism Peptides / chemistry RNA, Messenger / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R21 HL091344/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Peptides; 0/RNA, Messenger; 9007-92-5/Glucagon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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