Document Detail

Regulation of monocyte chemotactic protein-1 expression in human endometrial endothelial cells by sex steroids: a potential mechanism for leukocyte recruitment in endometriosis.
MedLine Citation:
PMID:  19933497     Owner:  NLM     Status:  MEDLINE    
The main aim of this study is to describe the in vivo temporal and spatial expression of monocyte chemotactic protein 1 (MCP-1) in human endometrial endothelial cells (HEECs) and to compare the in vitro regulation of MCP-1 expression by sex steroids in HEECs from women with or without endometriosis. Eutopic endometrial tissues and endometriosis implants were grouped according to the menstrual cycle phase and were examined by immunohistochemistry for MCP-1 expression. No significant difference was observed for MCP-1 immunoreactivity in the endothelial cells of eutopic endometrium of women with endometriosis when compared to endometrium of women without endometriosis and to endometriosis implants. For in vitro studies, the purity of cultured HEECs (90%-95%) was confirmed by immunocytochemistry using endothelium-specific markers CD31 and CD146. The effects of estradiol (5 x 10(- 8) mol/L), progesterone (10(-7) mol/L), or both on MCP-1 messenger RNA (mRNA) and protein levels were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and enzyme-linked immunosorbent serologic assay (ELISA), respectively. Sex steroids did not have significant effect on MCP-1 mRNA and protein expression in HEECs from women without endometriosis. However, we observed that the sex steroid treatment stimulated MCP-1 mRNA and protein expression in HEECs from women with endometriosis (P < .05). We postulate that the stimulation of chemokine expression by sex steroids in the endometrial endothelial cells in women with endometriosis may play a central role in recruiting mononuclear cells, therefore contributing to the inflammatory aspect of endometriosis.
Janelle Luk; Yasemin Seval; Murat Ulukus; Emine Cagnur Ulukus; Aydin Arici; Umit A Kayisli
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Publication Detail:
Type:  Journal Article     Date:  2009-11-20
Journal Detail:
Title:  Reproductive sciences (Thousand Oaks, Calif.)     Volume:  17     ISSN:  1933-7205     ISO Abbreviation:  Reprod Sci     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-12     Completed Date:  2010-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101291249     Medline TA:  Reprod Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  278-87     Citation Subset:  IM    
Section of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06520, USA.
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MeSH Terms
Cells, Cultured
Chemokine CCL2 / analysis,  genetics*
Endometriosis / immunology,  metabolism*
Endometrium / chemistry*
Endothelial Cells / chemistry
Enzyme-Linked Immunosorbent Assay
Estradiol / pharmacology
Gene Expression Regulation / drug effects*
Gonadal Steroid Hormones / pharmacology*
Leukocytes / physiology*
Middle Aged
Progesterone / pharmacology
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Chemokine CCL2; 0/Gonadal Steroid Hormones; 0/RNA, Messenger; 50-28-2/Estradiol; 57-83-0/Progesterone

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