Document Detail

Regulation of MT1-MMP activity by β-catenin in MDCK non-cancer and HT1080 cancer cells.
MedLine Citation:
PMID:  20589835     Owner:  NLM     Status:  MEDLINE    
Past studies on β-catenin in cancer cells focused on nuclear localized β-catenin and its involvement in the Wnt pathway. Our goal here was to investigate the function of β-catenin in both the cytoplasm and nucleus on the regulation of MT1-MMP expression and activity. We found that β-catenin in MDCK non-cancer cells inhibited the cell surface localization of MT1-MMP, and thus its proteolytic activity on pro-MMP2 activation, via direct interaction with the 18-amino-acid cytoplasmic tail of MT1-MMP in the cytoplasm. In contrast, β-catenin in HT1080 cancer cells enhanced the activity of MT1-MMP by entering the nucleus and activating transcription factor Tcf-4/Lef, and elevating the level of MT1-MMP protein. We also found that enhancement of cell growth in three-dimensional (3-D)/two-dimensional (2-D) type I collagen gels and of cell migration by MT1-MMP were inhibited by β-catenin in MDCK cells, whereas these functions were enhanced in HT1080 cells. In addition, regulation of MT1-MMP by β-catenin involved E-cadherin in MDCK cells and Wnt-3a in HT1080 cells. Taken together, our results present a differential effect of cytoplasmic and nuclear β-catenin on MT1-MMP activity in non-cancer cells versus cancer cells. These differences were most probably due to different subcellular locations and different involved pathways of β-catenin in these cells.
Ping Liu; Jianbo Yang; Jing Pei; Duanqing Pei; Michael J Wilson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  225     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-09-15     Completed Date:  2010-10-12     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  810-21     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley-Liss, Inc.
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MeSH Terms
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / metabolism
Cadherins / metabolism
Cell Line, Tumor
Cell Movement
Cell Nucleus / enzymology
Cell Proliferation
Colorectal Neoplasms / enzymology*,  pathology
Cytoplasm / enzymology
Enzyme Precursors / metabolism
Epithelial Cells / enzymology*
Gelatinases / metabolism
Gene Expression Regulation, Enzymologic
Kidney / cytology,  enzymology*
Matrix Metalloproteinase 14 / chemistry,  genetics,  metabolism*
Matrix Metalloproteinase 2 / metabolism
Protein Structure, Tertiary
RNA Interference
Transcription Factors / metabolism
Transcription, Genetic
Wnt Proteins / metabolism
Wnt3 Protein
Wnt3A Protein
beta Catenin / genetics,  metabolism*
Grant Support
CA114418-01A2/CA/NCI NIH HHS; R01 CA114418/CA/NCI NIH HHS
Reg. No./Substance:
0/Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; 0/CTNNB1 protein, human; 0/Cadherins; 0/Enzyme Precursors; 0/TCF4 protein, human; 0/Transcription Factors; 0/WNT3A protein, human; 0/Wnt Proteins; 0/Wnt3 Protein; 0/Wnt3A Protein; 0/beta Catenin; EC 3.4.24.-/Gelatinases; EC 3.4.24.-/progelatinase; EC protein, human; EC Metalloproteinase 2; EC protein, human; EC Metalloproteinase 14

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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