| Regulation of ICAM-1 expression in mouse macrophages. | |
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MedLine Citation:
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PMID: 10718114 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a mouse model of silica (SI) induced lung injury, SI exposure increases expression of intercellular adhesion molecule-1 (ICAM-1) on lung (alveolar/interstitial) macrophages and alveolar type II epithelial cells. To investigate the regulation of SI induced ICAM-1 expression on mouse macrophages, freshly isolated macrophages (alveolar, peritoneal) and macrophage cell lines (MH-S, RAW 264.7) were evaluated for ICAM-1 expression elicited by the particle silica (alpha quartz; 20 microg/ml; 6 microg/cm2) or the inflammatory cytokine, TNFalpha (20 ng/ml). TNFalpha significantly increased ICAM-1 expression in all cell types whereas SI elicited an increase in peritoneal macrophages (PM) and the cell line, MH-S. This pattern of increased expression was confirmed by immunocytochemistry. To investigate the regulation of ICAM-1 expression, PM were incubated with SI, TNFalpha or media concomitantly with anti-TNFalpha antibody, the antioxidant, NAC, or the iNOS synthase inhibitor, L-NAME. Both anti-TNFalpha and NAC, but not L-NAME, inhibited elicited (TNFalpha, SI) as well as constitutive (media) ICAM-1 expression. These data demonstrate that both inflammatory cytokines and inorganic particles can increase ICAM-1 expression on mouse macrophages and that this expression is mediated, in part, by TNFalpha and reactive oxygen species. |
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Authors:
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A K Hubbard; C Giardina |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Inflammation Volume: 24 ISSN: 0360-3997 ISO Abbreviation: Inflammation Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-04-13 Completed Date: 2000-04-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600105 Medline TA: Inflammation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 115-25 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Sciences, University of Connecticut, Storrs 06269, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology Animals Antibodies, Monoclonal / pharmacology Female Free Radical Scavengers / pharmacology Intercellular Adhesion Molecule-1 / biosynthesis* Macrophages / chemistry* Macrophages, Alveolar / chemistry Macrophages, Peritoneal / chemistry Mice Mice, Inbred C57BL Silicon Dioxide / pharmacology Tumor Cells, Cultured Tumor Necrosis Factor-alpha / immunology, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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R15ES09433/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Free Radical Scavengers; 0/Tumor Necrosis Factor-alpha; 126547-89-5/Intercellular Adhesion Molecule-1; 616-91-1/Acetylcysteine; 7631-86-9/Silicon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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