Document Detail


Regulation of the GnT-V promoter by transcription factor Ets-1 in various cancer cell lines.
MedLine Citation:
PMID:  10438459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although the precise role of oligosaccharides in metastasis is presently unknown, numerous studies suggest that the beta1-6 branching structure of N-linked oligosaccharides plays a role in tumor metastasis. N-Acetylglucosaminyltransferase V (GnT-V), which catalyzes the formation of the beta1-6 branch, therefore appears to play a crucial role in tumor metastasis. Recently, we demonstrated that the expression of the GnT-V gene is regulated by a transcriptional factor, Ets-1 (Kang, R., Saito, H., Ihara, Y., Miyoshi, E., Koyama, N., Sheng, Y., and Taniguchi, N. (1996) J. Biol. Chem. 271, 26706-26712). In this study, we report an investigation of the general requirement for Ets-1 in the expression of GnT-V in cancer cell lines. In 16 cancer cell lines, the levels of GnT-V mRNA were closely correlated with ets-1 expression (r = 0.97; p < 0.0001). An increase in ets-1 levels by transfection of its cDNA led to an enhancement in GnT-V expression in cells that normally expressed low levels of ets-1. In contrast, the transfection of dominant negative ets-1 into cells that express high levels of ets-1 resulted in a decrease in GnT-V expression. Although Ets-1 cooperates with c-Jun in certain gene expressions, this was not the case in the regulation of the GnT-V gene. These results suggest that Ets-1 plays a significant role in regulating the expression of GnT-V in a variety of cancers and might be involved in the potential for malignancy via the action of GnT-V.
Authors:
J H Ko; E Miyoshi; K Noda; A Ekuni; R Kang; Y Ikeda; N Taniguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-01     Completed Date:  1999-09-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  22941-8     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Osaka University Medical School, Room B1, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA Primers
Gene Expression Regulation, Enzymologic*
Humans
N-Acetylglucosaminyltransferases / genetics*
Promoter Regions, Genetic*
Proto-Oncogene Protein c-ets-1
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins c-ets
Transcription Factor AP-1 / genetics
Transcription Factors / metabolism*
Transfection
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/DNA Primers; 0/ETS1 protein, human; 0/Proto-Oncogene Protein c-ets-1; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-ets; 0/Transcription Factor AP-1; 0/Transcription Factors; EC 2.4.1.-/N-Acetylglucosaminyltransferases; EC 2.4.1.155/alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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