Document Detail


Regulation of GLUT1 gene transcription by the serine/threonine kinase Akt1.
MedLine Citation:
PMID:  10400647     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We used mouse hepatoma (Hepa1c1c7) cells to study the role of the serine/threonine kinase Akt in the induction of GLUT1 gene expression. In order to selectively turn on the Akt kinase cascade, we expressed a hydroxytamoxifen-regulatable form of Akt (myristoylated Akt1 estrogen receptor chimera (MER-Akt1)) in the Hepa1c1c7 cells; we verified that hydroxytamoxifen stimulates MER-Akt1 activity to a similar extent as the activation of endogenous Akt by insulin. Our studies reveal that stimulation of MER-Akt1 by hydroxytamoxifen induces GLUT1 mRNA and protein accumulation to levels comparable to that induced by insulin; therefore, activation of the Akt cascade suffices to induce GLUT1 gene expression in this cell system. Furthermore, expression of a kinase-inactive Akt mutant partially inhibits the response of the GLUT1 gene to insulin. Additional studies reveal that the induction of GLUT1 mRNA by Akt and by insulin reflects increased mRNA synthesis and not decreased mRNA degradation. Our findings imply that the GLUT1 gene responds to insulin at the transcriptional level and that Akt mediates a step in the activation of GLUT1 gene expression in this system.
Authors:
A Barthel; S T Okino; J Liao; K Nakatani; J Li; J P Whitlock; R A Roth
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-08-19     Completed Date:  1999-08-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  20281-6     Citation Subset:  IM    
Affiliation:
Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, California 94305, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Gene Expression Regulation* / drug effects
Glucose Transporter Type 1
Insulin / pharmacology
Liver Neoplasms, Experimental / enzymology,  genetics
Mice
Monosaccharide Transport Proteins / genetics*
Protein-Serine-Threonine Kinases / metabolism*
Proto-Oncogene Proteins*
Proto-Oncogene Proteins c-akt
RNA, Messenger / genetics
Transcription, Genetic*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
DK 34926/DK/NIDDK NIH HHS; ES08655/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Glucose Transporter Type 1; 0/Monosaccharide Transport Proteins; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; 0/Slc2a1 protein, mouse; 11061-68-0/Insulin; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/Proto-Oncogene Proteins c-akt

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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