Document Detail

Regulation of DUOX by the Galphaq-phospholipase Cbeta-Ca2+ pathway in Drosophila gut immunity.
MedLine Citation:
PMID:  19289084     Owner:  NLM     Status:  MEDLINE    
All metazoan guts are in constant contact with diverse food-borne microorganisms. The signaling mechanisms by which the host regulates gut-microbe interactions, however, are not yet clear. Here, we show that phospholipase C-beta (PLCbeta) signaling modulates dual oxidase (DUOX) activity to produce microbicidal reactive oxygen species (ROS) essential for normal host survival. Gut-microbe contact rapidly activates PLCbeta through Galphaq, which in turn mobilizes intracellular Ca(2+) through inositol 1,4,5-trisphosphate generation for DUOX-dependent ROS production. PLCbeta mutant flies had a short life span due to the uncontrolled propagation of an essential nutritional microbe, Saccharomyces cerevisiae, in the gut. Gut-specific reintroduction of the PLCbeta restored efficient DUOX-dependent microbe-eliminating capacity and normal host survival. These results demonstrate that the Galphaq-PLCbeta-Ca(2+)-DUOX-ROS signaling pathway acts as a bona fide first line of defense that enables gut epithelia to dynamically control yeast during the Drosophila life cycle.
Eun-Mi Ha; Kyung-Ah Lee; Seon Hwa Park; Sung-Hee Kim; Hyuck-Jin Nam; Hyo-Young Lee; Dongmin Kang; Won-Jae Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental cell     Volume:  16     ISSN:  1878-1551     ISO Abbreviation:  Dev. Cell     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-17     Completed Date:  2009-04-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101120028     Medline TA:  Dev Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  386-97     Citation Subset:  IM    
Division of Life and Pharmaceutical Science, Ewha Woman's University, Seoul 120-750, South Korea.
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MeSH Terms
Animals, Genetically Modified
Calcium Signaling
Cell Line
Digestive System / immunology,  metabolism,  microbiology
Drosophila / genetics,  immunology*,  metabolism*
Drosophila Proteins / genetics,  metabolism*
GTP-Binding Protein alpha Subunits, Gq-G11 / genetics,  metabolism*
Immunity, Innate
Inositol 1,4,5-Trisphosphate / biosynthesis
Models, Biological
NADPH Oxidase / metabolism*
Phospholipase C beta / genetics,  metabolism*
Reactive Oxygen Species / metabolism
Saccharomyces cerevisiae / growth & development,  immunology
Signal Transduction
Reg. No./Substance:
0/Drosophila Proteins; 0/Reactive Oxygen Species; 0/dual oxidase, Drosophila; 85166-31-0/Inositol 1,4,5-Trisphosphate; EC Oxidase; EC C beta; EC Protein alpha Subunits, Gq-G11

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