Document Detail


Regulation of DNA damage response and cell cycle in radiation-resistant HL60 myeloid leukemia cells.
MedLine Citation:
PMID:  22576796     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The acquisition of resistance to radiation has been a matter of concern in clinical cases. However, mechanisms underlying the acquisition of radiation resistance are yet to be elucidated. We established a radiation-resistant strain (Res-HL60 cells) from HL60 leukemic cells and investigated their response to radiation. Res-HL60 cells not only proliferated on the fifth day after radiation but also had a high survival rate in a clonogenic assay. Although Chk1 was activated in HL60 cells after irradiation, the expression levels of Chk1 in Res-HL60 cells decreased. There were few differences between the two cell lines with regard to Chk2 activity. Res-HL60 cells show prolonged G2/M arrest and an early decrease in the extent of DNA damage. However, inhibitors against ATM/ATR and DNA-dependent protein kinase (DNA-PK) both abrogated the radiation resistance capacity of the cells. These results reveal that radiation-resistant strains have a high repair capacity, and inhibition of the repair system at an early stage is an effective strategy in the second round of radiation therapy.
Authors:
Masaharu Hazawa; Yoichiro Hosokawa; Satoru Monzen; Hironori Yoshino; Ikuo Kashiwakura
Related Documents :
8422986 - Morphogenesis in drosophila requires nonmuscle myosin heavy chain function.
8070636 - Cytoplasmic ca2+ and h+ concentrations determine cell fate in dictyostelium discoideum.
14595116 - Two phases of actin polymerization display different dependencies on pi(3,4,5)p3 accumu...
25417056 - Elevated expression of secreted autocrine growth factor progranulin increases cervical ...
21311236 - A kinase's work is never done: rad53 monitors chromatin near replication origins.
11776096 - Aristolochic acid i-induced apoptosis in llc-pk1 cells and amelioration of the apoptoti...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-20
Journal Detail:
Title:  Oncology reports     Volume:  28     ISSN:  1791-2431     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-5-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  -    
Other Details:
Languages:  ENG     Pagination:  55-61     Citation Subset:  -    
Affiliation:
Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University, 66-1 Honcho, Hirosaki, Aomori 036-8564, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Antiproliferative action of metformin in human lung cancer cell lines.
Next Document:  Downregulation of hepatoma-derived growth factor suppresses the malignant phenotype of U87 human gli...