Document Detail

Regulation of DNA damage response and cell cycle in radiation-resistant HL60 myeloid leukemia cells.
MedLine Citation:
PMID:  22576796     Owner:  NLM     Status:  Publisher    
The acquisition of resistance to radiation has been a matter of concern in clinical cases. However, mechanisms underlying the acquisition of radiation resistance are yet to be elucidated. We established a radiation-resistant strain (Res-HL60 cells) from HL60 leukemic cells and investigated their response to radiation. Res-HL60 cells not only proliferated on the fifth day after radiation but also had a high survival rate in a clonogenic assay. Although Chk1 was activated in HL60 cells after irradiation, the expression levels of Chk1 in Res-HL60 cells decreased. There were few differences between the two cell lines with regard to Chk2 activity. Res-HL60 cells show prolonged G2/M arrest and an early decrease in the extent of DNA damage. However, inhibitors against ATM/ATR and DNA-dependent protein kinase (DNA-PK) both abrogated the radiation resistance capacity of the cells. These results reveal that radiation-resistant strains have a high repair capacity, and inhibition of the repair system at an early stage is an effective strategy in the second round of radiation therapy.
Masaharu Hazawa; Yoichiro Hosokawa; Satoru Monzen; Hironori Yoshino; Ikuo Kashiwakura
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-20
Journal Detail:
Title:  Oncology reports     Volume:  28     ISSN:  1791-2431     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-5-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  -    
Other Details:
Languages:  ENG     Pagination:  55-61     Citation Subset:  -    
Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University, 66-1 Honcho, Hirosaki, Aomori 036-8564, Japan.
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