| Regulation of cytosolic and mitochondrial oxidation via malate-aspartate shuttle: an observation using dynamic ¹³C NMR spectroscopy. | |
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MedLine Citation:
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PMID: 21445786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The malate-aspartate (M-A) shuttle provides an important mechanism of metabolic communication between the cytosol and the mitochondria. In this study, dynamic (13)C NMR spectroscopy was combined with a multi-domain model of cardiac metabolism for direct quantification of metabolic fluxes through the tricarboxylic acid (TCA) cycle (VTCA) and the M-A shuttle (VM-A) in intact heart. The sensitivity of this approach to altered M-A shuttle activity was examined at different cytosolic redox states. Dynamic (13)C NMR spectra were acquired from isolated rat hearts perfused with (13)C labeled fatty acid at either low (fatty acid only) or high cytosolic redox state induced by exogenous glucose and lactate. VTCA and VM-A were determined by least-square fitting of the model to NMR data. Our results showed that while VTCA was similar, VM-A increased by 75% at high cytosolic redox state. Therefore, our proposed method provides the opportunity for direct quantification of metabolic communication between subcellular compartments via the M-A shuttle. |
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Authors:
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Ming Lu; Suhanti Banerjee; Gerald M Saidel; Xin Yu |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Advances in experimental medicine and biology Volume: 701 ISSN: 0065-2598 ISO Abbreviation: Adv. Exp. Med. Biol. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-03-29 Completed Date: 2011-08-16 Revised Date: 2012-04-09 |
Medline Journal Info:
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Nlm Unique ID: 0121103 Medline TA: Adv Exp Med Biol Country: United States |
Other Details:
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Languages: eng Pagination: 185-92 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspartic Acid / metabolism* Biological Transport Cell Respiration Citric Acid Cycle / physiology Cytosol / metabolism* Heart / physiology Lactates / metabolism Magnetic Resonance Spectroscopy* Malates / metabolism* Male Mitochondria / metabolism* Oxidation-Reduction Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL073315-06A2/HL/NHLBI NIH HHS; R01 HL073315-07/HL/NHLBI NIH HHS; R01 HL086935-03W1/HL/NHLBI NIH HHS; R01 HL086935-04/HL/NHLBI NIH HHS; R01HL73315/HL/NHLBI NIH HHS; R01HL86935/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Lactates; 0/Malates; 56-84-8/Aspartic Acid; 6915-15-7/malic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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