Document Detail


Regulation of cytosolic and mitochondrial oxidation via malate-aspartate shuttle: an observation using dynamic ¹³C NMR spectroscopy.
MedLine Citation:
PMID:  21445786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The malate-aspartate (M-A) shuttle provides an important mechanism of metabolic communication between the cytosol and the mitochondria. In this study, dynamic (13)C NMR spectroscopy was combined with a multi-domain model of cardiac metabolism for direct quantification of metabolic fluxes through the tricarboxylic acid (TCA) cycle (VTCA) and the M-A shuttle (VM-A) in intact heart. The sensitivity of this approach to altered M-A shuttle activity was examined at different cytosolic redox states. Dynamic (13)C NMR spectra were acquired from isolated rat hearts perfused with (13)C labeled fatty acid at either low (fatty acid only) or high cytosolic redox state induced by exogenous glucose and lactate. VTCA and VM-A were determined by least-square fitting of the model to NMR data. Our results showed that while VTCA was similar, VM-A increased by 75% at high cytosolic redox state. Therefore, our proposed method provides the opportunity for direct quantification of metabolic communication between subcellular compartments via the M-A shuttle.
Authors:
Ming Lu; Suhanti Banerjee; Gerald M Saidel; Xin Yu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  701     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-03-29     Completed Date:  2011-08-16     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  185-92     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspartic Acid / metabolism*
Biological Transport
Cell Respiration
Citric Acid Cycle / physiology
Cytosol / metabolism*
Heart / physiology
Lactates / metabolism
Magnetic Resonance Spectroscopy*
Malates / metabolism*
Male
Mitochondria / metabolism*
Oxidation-Reduction
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
R01 HL073315/HL/NHLBI NIH HHS; R01 HL073315-06A2/HL/NHLBI NIH HHS; R01 HL073315-07/HL/NHLBI NIH HHS; R01 HL086935/HL/NHLBI NIH HHS; R01 HL086935-03W1/HL/NHLBI NIH HHS; R01 HL086935-04/HL/NHLBI NIH HHS; R01HL73315/HL/NHLBI NIH HHS; R01HL86935/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Lactates; 0/Malates; 30KYC7MIAI/Aspartic Acid; 6915-15-7/malic acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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