Document Detail


Regulation of Cl- secretion in seawater fish (Dicentrarchus labrax) gill respiratory cells in primary culture.
MedLine Citation:
PMID:  10087336     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Primary cultures of sea bass (Dicentrarchus labrax) gill cells grown on permeable membranes form a highly differentiated tight epithelium composed of respiratory-like cells. This preparation was also found to provide a functional model for investigating the hormonal regulation of Cl- secretion. 2. In control conditions, i.e. in the absence of hormones or other stimuli, the cultured epithelium showed a short-circuit current (Isc) of 8.8 +/- 0.4 microA cm-2, a transepithelial potential (Vt) of 28.6 +/- 0.6 mV (serosal side positive), and a transepithelial resistance (Rt) of 5026 +/- 127 Omega cm2. Addition of 50 nM PGE2 caused a stimulation of Isc, Vt and transepithelial conductance, Gt. The increase in Isc was probably due to the elevation in Cl- secretion, since it could be correlated with the stimulation of serosal to mucosal 36Cl- flux. Application of the neurohypophyseal peptide arginine vasotocin (AVT; 50 nM) or the beta-adrenergic agonist isoproterenol (isoprenaline; 0. 5 microM) evoked a stimulation in Cl- secretion, as was shown by the increases in Isc and Gt. The excitatory effect of isoproterenol followed by the inhibitory action of propranolol, a beta-adrenergic antagonist, suggested the presence of beta-adrenergic receptors. Noradrenaline (0.1 microM) elicited a reduction in Isc, Vt and Gt, which was counterbalanced by the addition of phentolamine, an alpha-adrenergic antagonist. This suggested an activation of alpha-adrenergic receptors. 3. This study provides evidence for hormonal control of the Cl- secretion in sea bass gill respiratory cells in culture, involving AVT, prostaglandin (PGE2), and beta- and alpha-adrenergic receptors.
Authors:
M Avella; P Part; J Ehrenfeld
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of physiology     Volume:  516 ( Pt 2)     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-06-29     Completed Date:  1999-06-29     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  353-63     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie Cellulaire et Moleculaire, UMR CNRS 6548, Universite de Nice Sophia-Antipolis, Faculte des Sciences, Parc Valrose, 06108 Nice Cedex 2, France. avella@unice.fr
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MeSH Terms
Descriptor/Qualifier:
Animals
Bass / metabolism*
Catecholamines / pharmacology,  physiology
Cells, Cultured
Chloride Channels / metabolism
Chlorides / metabolism*
Dinoprostone / pharmacology
Electrophysiology
Gills / cytology,  drug effects,  metabolism*
Hormones / metabolism
Kinetics
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Oxygen Consumption / drug effects,  physiology
Patch-Clamp Techniques
Vasotocin / pharmacology
Chemical
Reg. No./Substance:
0/Catecholamines; 0/Chloride Channels; 0/Chlorides; 0/Hormones; 363-24-6/Dinoprostone; 9034-50-8/Vasotocin
Comments/Corrections

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