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Regulation of the Cinnamate 4-Hydroxylase (CYP73A1) in Jerusalem Artichoke Tubers in Response to Wounding and Chemical Treatments.
MedLine Citation:
PMID:  12223655     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
trans-Cinnamate 4-hydroxylase (C4H) is a plant-specific cytochrome (P450) that is encoded by the gene CYP73A and catalyzes the second step of the multibranched phenylpropanoid pathway. Increases in C4H activity in response to physical and chemical stresses have been well documented, but the mechanism of these increases has never been studied in detail. This paper reports on the regulatory mechanism controlling C4H activity in Jerusalem artichoke (Helianthus tuberosus) tubers in response to wounding and chemical treatments. We compared induction of C4H and other P450-catalyzed activities. C4H was moderately induced by chemicals relative to other P450s. Increases in enzyme activity, C4H protein, and transcripts were quantified and compared in tuber tissue 48 h after wounding and chemical treatments. Our data suggest that induction of the enzyme activity results primarily from gene activation. Time-course experiments were performed after wounding and aminopyrine treatment. Compared with wounded tissues, aminopyrine triggered an additional and delayed peak of transcript accumulation. The timing of the induced changes in activity, protein, and transcripts confirms that C4H induction results primarily from an increase in CYP73A1 mRNA, in both wounded and aminopyrine-treated tissues. However, posttranscriptional mechanisms might also contribute to the regulation of C4H activity.
Authors:
Y. Batard; M. Schalk; M. A. Pierrel; A. Zimmerlin; F. Durst; D. Werck-Reichhart
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Plant physiology     Volume:  113     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  2002-Sep-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  951-959     Citation Subset:  -    
Affiliation:
Departement d'Enzymologie Cellulaire et Moleculaire, Institut de Biologie Moleculaire des Plantes, Centre National de la Recherche Scientifique, 28 Rue Goethe, 67000 Strasbourg, France.
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