| Regulation of the Chlamydomonas cell cycle by a stable, chromatin-associated retinoblastoma tumor suppressor complex. | |
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MedLine Citation:
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PMID: 20978220 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined the cell cycle dynamics of the retinoblastoma (RB) protein complex in the unicellular alga Chlamydomonas reinhardtii that has single homologs for each subunit-RB, E2F, and DP. We found that Chlamydomonas RB (encoded by MAT3) is a cell cycle-regulated phosphoprotein, that E2F1-DP1 can bind to a consensus E2F site, and that all three proteins interact in vivo to form a complex that can be quantitatively immunopurified. Yeast two-hybrid assays revealed the formation of a ternary complex between MAT3, DP1, and E2F1 that requires a C-terminal motif in E2F1 analogous to the RB binding domain of plant and animal E2Fs. We examined the abundance of MAT3/RB and E2F1-DP1 in highly synchronous cultures and found that they are synthesized and remain stably associated throughout the cell cycle with no detectable fraction of free E2F1-DP1. Consistent with their stable association, MAT3/RB and DP1 are constitutively nuclear, and MAT3/RB does not require DP1-E2F1 for nuclear localization. In the nucleus, MAT3/RB remains bound to chromatin throughout the cell cycle, and its chromatin binding is mediated through E2F1-DP1. Together, our data show that E2F-DP complexes can regulate the cell cycle without dissociation of their RB-related subunit and that other changes may be sufficient to convert RB-E2F-DP from a cell cycle repressor to an activator. |
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Authors:
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Bradley J S C Olson; Michael Oberholzer; Yubing Li; James M Zones; Harjivan S Kohli; Katerina Bisova; Su-Chiung Fang; Jill Meisenhelder; Tony Hunter; James G Umen |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-10-26 |
Journal Detail:
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Title: The Plant cell Volume: 22 ISSN: 1532-298X ISO Abbreviation: Plant Cell Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-11-30 Completed Date: 2011-04-14 Revised Date: 2012-05-07 |
Medline Journal Info:
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Nlm Unique ID: 9208688 Medline TA: Plant Cell Country: United States |
Other Details:
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Languages: eng Pagination: 3331-47 Citation Subset: IM |
Affiliation:
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Plant Molecular and Cellular Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AAK68064; AAV41810; AAV68604; DQ417491; DQ417492; GU784915; U54830; RefSeq/NP_566417; XP_002948442; XP_002955099 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle* Chlamydomonas / cytology*, genetics, metabolism Chromatin / metabolism* E2F Transcription Factors / genetics, metabolism Gene Expression Regulation, Plant Molecular Sequence Data Phosphoproteins / genetics, metabolism Phosphorylation Plant Proteins / metabolism* Protozoan Proteins / genetics, metabolism* Recombinant Proteins / genetics, metabolism Retinoblastoma Protein / genetics, metabolism* Transcription Factor DP1 / genetics, metabolism Two-Hybrid System Techniques |
| Grant Support | |
ID/Acronym/Agency:
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CA-832683/CA/NCI NIH HHS; GM086037/GM/NIGMS NIH HHS; R01 CA082683-13/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/E2F Transcription Factors; 0/Phosphoproteins; 0/Plant Proteins; 0/Protozoan Proteins; 0/Recombinant Proteins; 0/Retinoblastoma Protein; 0/Transcription Factor DP1; 0/mat3 protein, Chlamydomonas |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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