| Regulation of CXC chemokine receptor 4-mediated migration by the Tec family tyrosine kinase ITK. | |
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MedLine Citation:
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PMID: 15123627 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chemokines are critical in controlling lymphocyte traffic and migration. The CXC chemokine CXCL12/SDF-1alpha interacts with its receptor CXCR4 to induce the migration of a number of different cell types. Although an understanding of the physiological functions of this chemokine is emerging, the mechanism by which it regulates T cell migration is still unclear. We show here that the Tec family kinase ITK is activated rapidly following CXCL12/SDF-1alpha stimulation, and this requires Src and phosphatidylinositol 3-kinase activities. ITK regulates the ability of CXCL12/SDF-1alpha to induce T cell migration as overexpression of wild-type ITK-enhanced migration, and T cells lacking ITK exhibit reduced migration as well as adhesion in response to CXCL12/SDF-1alpha. Further analysis suggests that ITK may regulate CXCR4-mediated migration and adhesion by altering the actin cytoskeleton, as ITK null T cells were significantly defective in CXCL12/SDF-1a-mediated actin polymerization. Our data suggest that ITK may regulate the ability of CXCR4 to induce T cell migration. |
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Authors:
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Angela M Fischer; Jason C Mercer; Archana Iyer; Melanie J Ragin; Avery August |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Date: 2004-04-26 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 279 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-07-05 Completed Date: 2004-08-24 Revised Date: 2012-06-01 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 29816-20 Citation Subset: IM |
Affiliation:
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Department of Veterinary Science, Pathobiology Graduate Program, Pennsylvania State University, University Park, Pennsylvania 16802, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Movement / physiology* Chemokine CXCL12 Chemokines, CXC / metabolism Enzyme Activation Humans Jurkat Cells / metabolism* Mice Mice, Inbred C57BL Phosphatidylinositol 3-Kinases / metabolism Protein-Tyrosine Kinases / genetics, metabolism* Receptors, CXCR4 / metabolism* Signal Transduction / physiology src-Family Kinases / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01-AI51626/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CXCL12 protein, human; 0/Chemokine CXCL12; 0/Chemokines, CXC; 0/Cxcl12 protein, mouse; 0/Receptors, CXCR4; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.2/emt protein-tyrosine kinase; EC 2.7.10.2/src-Family Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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