| Regulation of 5-aminolevulinate synthase in mouse erythroleukemic cells is different from that in liver. | |
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MedLine Citation:
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PMID: 3166455 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have measured the transcriptional gene activity of 5-aminolevulinate synthase, the first enzyme of the heme biosynthetic pathway, together with corresponding mRNA and protein levels in mouse erythroleukemic cells induced to differentiate with dimethyl sulfoxide. When the heme biosynthetic pathway was blocked by succinylacetone there was a large increase in both 5-aminolevulinate synthase activity and protein levels, and this was reversed by the addition of exogenous hemin. Transcriptional activity of the 5-aminolevulinate synthase gene and mRNA levels were both significantly increased during differentiation of cells by dimethyl sulfoxide but were not markedly altered by succinylacetone or hemin treatment. The results demonstrate that levels of 5-aminolevulinate synthase in mouse erythroleukemic cells are regulated by a significant post-transcriptional mechanism possibly at the translational level. Evidence is also presented for a less significant post-transcriptional control by heme of mRNA levels for 5-aminolevulinate synthase. These results indicate that the regulation of 5-aminolevulinate synthase in differentiating erythroid cells is complex but differs from that in liver cells where heme controls the level of 5-aminolevulinate synthase by acting primarily to inhibit gene transcription. |
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Authors:
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C J Elferink; S Sassa; B K May |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 263 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1988 Sep |
Date Detail:
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Created Date: 1988-10-19 Completed Date: 1988-10-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 13012-6 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Stanford University School of Medicine, California 94305. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5-Aminolevulinate Synthetase
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genetics,
metabolism* Animals Hemin / pharmacology Heptanoates / pharmacology Leukemia, Erythroblastic, Acute / enzymology* Liver / enzymology* Mice RNA, Messenger / metabolism Transcription, Genetic / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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DK-32890/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Heptanoates; 0/RNA, Messenger; 16009-13-5/Hemin; 51568-18-4/succinylacetone; EC 2.3.1.37/5-Aminolevulinate Synthetase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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