| Regulation of [3H]norepinephrine release by N-methyl-D-aspartate receptors in minislices from the dentate gyrus and the CA1-CA3 area of the rat hippocampus. | |
| | |
MedLine Citation:
|
PMID: 8267648 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
It has been reported previously that N-methyl-D-aspartic acid induces a significant release of [3H]norepinephrine preaccumulated in slices from the hippocampus. In the present study, we investigated whether there are regional differences in the hippocampus regarding this N-methyl-D-aspartate effect. In the absence of Mg2+, N-methyl-D-aspartate (10-200 microM) induced the release of [3H]norepinephrine from superfused minislices containing the dentate gyrus area or the CA1-CA3 region of the hippocampus. Such N-methyl-D-aspartate effects on [3H]norepinephrine release were significantly higher in the dentate gyrus than in the CA1-CA3 area. The N-methyl-D-aspartate effects in both hippocampal areas were also reduced significantly by D-2-amino-5-phosphonovaleric acid (50 microM), an antagonist of the N-methyl-D-aspartate receptor, and by tetrodotoxin, a blocker of the voltage-dependent Na+ channels. The extent of this reduction was the same in the dentate gyrus and the CA1-CA3 area. Further experiments, conducted in the presence of Mg2+, demonstrated that N-methyl-D-aspartic acid increased K(+)-induced release of [3H]norepinephrine from dentate gyrus minislices but not from the CA1-CA3 area. The results are consistent with the existence of a higher density and/or different subtypes of N-methyl-D-aspartate receptors modulating [3H]norepinephrine release in the dentate gyrus as compared with the CA1-CA3 hippocampal area. |
| | |
Authors:
|
M E Andrés; G Bustos; K Gysling |
Related Documents
:
|
3033558 - Single sensory neurons activate excitatory amino acid receptors in the lamprey spinal c... 21664238 - Corepressor effect on androgen receptor activity varies with the length of the cag enco... 19708978 - Guanidino compounds as uremic (neuro)toxins. 10717438 - Perfusion of the hypothalamic paraventricular nucleus with n-methyl-d-aspartate produce... 1373798 - Late-stage spermatids are characterized by expression of the "liver-specific" asialogly... 7901348 - Basic fgf regulates the expression of a functional 71 kda nmda receptor protein that me... |
Publication Detail:
|
Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemical pharmacology Volume: 46 ISSN: 0006-2952 ISO Abbreviation: Biochem. Pharmacol. Publication Date: 1993 Dec |
Date Detail:
|
Created Date: 1994-01-24 Completed Date: 1994-01-24 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 1983-7 Citation Subset: IM |
Affiliation:
|
Department of Cell and Molecular Biology, Faculty of Biological Sciences, Catholic University of Chile, Santiago. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Hippocampus / metabolism* Limbic System / metabolism* Male N-Methylaspartate / antagonists & inhibitors, pharmacology Norepinephrine / secretion* Potassium / pharmacology Rats Rats, Sprague-Dawley Receptors, N-Methyl-D-Aspartate / metabolism* Tritium |
| Chemical | |
Reg. No./Substance:
|
0/Receptors, N-Methyl-D-Aspartate; 10028-17-8/Tritium; 51-41-2/Norepinephrine; 6384-92-5/N-Methylaspartate; 7440-09-7/Potassium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Validation of 4-nitrophenol as an in vitro substrate probe for human liver CYP2E1 using cDNA express...
Next Document: Combination studies with 3'-azido-3'-deoxythymidine (AZT) plus ICI D1694. Cytotoxic and biochemical ...