Document Detail

Regulation of [3H]norepinephrine release by N-methyl-D-aspartate receptors in minislices from the dentate gyrus and the CA1-CA3 area of the rat hippocampus.
MedLine Citation:
PMID:  8267648     Owner:  NLM     Status:  MEDLINE    
It has been reported previously that N-methyl-D-aspartic acid induces a significant release of [3H]norepinephrine preaccumulated in slices from the hippocampus. In the present study, we investigated whether there are regional differences in the hippocampus regarding this N-methyl-D-aspartate effect. In the absence of Mg2+, N-methyl-D-aspartate (10-200 microM) induced the release of [3H]norepinephrine from superfused minislices containing the dentate gyrus area or the CA1-CA3 region of the hippocampus. Such N-methyl-D-aspartate effects on [3H]norepinephrine release were significantly higher in the dentate gyrus than in the CA1-CA3 area. The N-methyl-D-aspartate effects in both hippocampal areas were also reduced significantly by D-2-amino-5-phosphonovaleric acid (50 microM), an antagonist of the N-methyl-D-aspartate receptor, and by tetrodotoxin, a blocker of the voltage-dependent Na+ channels. The extent of this reduction was the same in the dentate gyrus and the CA1-CA3 area. Further experiments, conducted in the presence of Mg2+, demonstrated that N-methyl-D-aspartic acid increased K(+)-induced release of [3H]norepinephrine from dentate gyrus minislices but not from the CA1-CA3 area. The results are consistent with the existence of a higher density and/or different subtypes of N-methyl-D-aspartate receptors modulating [3H]norepinephrine release in the dentate gyrus as compared with the CA1-CA3 hippocampal area.
M E Andrés; G Bustos; K Gysling
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  46     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-01-24     Completed Date:  1994-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1983-7     Citation Subset:  IM    
Department of Cell and Molecular Biology, Faculty of Biological Sciences, Catholic University of Chile, Santiago.
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MeSH Terms
Hippocampus / metabolism*
Limbic System / metabolism*
N-Methylaspartate / antagonists & inhibitors,  pharmacology
Norepinephrine / secretion*
Potassium / pharmacology
Rats, Sprague-Dawley
Receptors, N-Methyl-D-Aspartate / metabolism*
Reg. No./Substance:
0/Receptors, N-Methyl-D-Aspartate; 10028-17-8/Tritium; 51-41-2/Norepinephrine; 6384-92-5/N-Methylaspartate; 7440-09-7/Potassium

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