Document Detail

Regulation of the 12-O-tetradecanoyl-phorbol-13-acetate-induced inhibition of intercellular communication.
MedLine Citation:
PMID:  8417617     Owner:  NLM     Status:  MEDLINE    
Effects of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and protein kinase C (PKC) inhibitors on cell-cell communication were studied in a normal rat liver cell line, clone 9. Communication was observed and quantitated with microspectofluorometric and image analysis techniques following scrape-loading of the cells with lucifer yellow. Lucifer yellow migrated as far as ten cells away from the scraped edge in control populations. Two minute TPA (25-50 micrograms/ml) treatment inhibited dye movement such that the dye remained mainly in the cells at the cut edge. The TPA-induced inhibition of cell-cell communication could be partially blocked by 15 min treatment of the cell populations with the PKC inhibitors trifluoperazine (30 micrograms/ml), staurosporine (2 x 10(-8) or 2 x 10(-6) M), sangivamycin (15 or 200 microM), or a PKC inhibitor peptide (20 micrograms/ml) scraped in at the same time as lucifer yellow. Normal communication was observed in cultures treated only with PKC inhibitors. Lower concentrations of TPA (50 ng/ml-1 micrograms/ml) used for 2 min did not inhibit dye communication. Our results demonstrate the phorbol ester-induced interruption of cell-cell communication. The inhibition of PKC by inhibitors eliminates the effect of TPA on communication. Our data are consistent with a role of PKC in the control of junctional communication.
S A Murray; H K Plummer; E E Leonard; P Deshmukh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Anatomical record     Volume:  235     ISSN:  0003-276X     ISO Abbreviation:  Anat. Rec.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-01-28     Completed Date:  1993-01-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370540     Medline TA:  Anat Rec     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-11     Citation Subset:  IM    
Department of Neurobiology, University of Pittsburgh School of Medicine, Pennsylvania 15261.
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MeSH Terms
Alkaloids / pharmacology
Cell Communication / drug effects*
Cell Line
Image Processing, Computer-Assisted
Liver / cytology,  drug effects*
Microscopy, Fluorescence
Protein Kinase C / antagonists & inhibitors,  pharmacology
Pyrimidine Nucleosides / pharmacology
Tetradecanoylphorbol Acetate / pharmacology*
Trifluoperazine / pharmacology*
Reg. No./Substance:
0/Alkaloids; 0/Isoquinolines; 0/Pyrimidine Nucleosides; 117-89-5/Trifluoperazine; 16561-29-8/Tetradecanoylphorbol Acetate; 18417-89-5/sangivamycin; 62996-74-1/Staurosporine; 77944-88-8/lucifer yellow; EC Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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