Document Detail


Regulating potential in development of a direct developing echinoid, Peronella japonica.
MedLine Citation:
PMID:  11148453     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regulating potential along the animal-vegetal axis of a direct developing echinoid, Peronella japonica, was investigated using LiCl. Animal caps isolated from 16-cell stage P. japonica embryos developed to permanent blastulae with an amniotic cavity. Treatment of animal caps with LiCl induced them to vegetalize with differentiation of the endoderm and subsequently develop into pluteus-like larvae. The larvae derived from the LiCl-treated animal caps were able to metamorphose and establish an adult body plan. A considerable fraction of whole embryos treated with LiCl exogastrulated and/or evaginated an amniotic cavity. The timing of the sensitivity to LiCl-mediated induction of evagination of the amniotic cavity was earlier than that for exogastrulation. Peronella japonica embryos became sensitive to LiCl induction of exogastrulation later than embryos of indirect developers. Some larvae with evaginated archenteron and/or evaginated amniotic cavity had metamorphic potential. These results suggest that LiCl can induce both vegetalization and evagination of invaginating structures. The present study is the first to show the potential of the presumptive ectoderm region to regulate the establishment of the adult body plan without any influence from other blastomeres, revealing that the regulating potential of sea urchin embryos is much larger than previously thought.
Authors:
C Kitazawa; S Amemiya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development, growth & differentiation     Volume:  43     ISSN:  0012-1592     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  73-82     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1, Hongo, Bunkyo-Ku, Tokyo 113-0033, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dose-Response Relationship, Drug
Ectoderm / metabolism
Fertilization / physiology
Gene Expression Regulation, Developmental*
Lithium Chloride / pharmacology
Microscopy, Electron, Scanning
Models, Biological
Sea Urchins / embryology*,  ultrastructure
Time Factors
Chemical
Reg. No./Substance:
7447-41-8/Lithium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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