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FAT/CD36 Regulates PEPCK Expression in Adipose Tissue.
MedLine Citation:
PMID:  23302781     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
FAT/CD36 has been extensively studied for its role in facilitating fatty acid uptake. Recent findings have also demonstrated that this protein regulates adipocyte lipolysis and may modulate fatty acid re-esterification. As FAT/CD36 has been shown to control the expression of genes involved in fatty acid oxidation in adipocytes we reasoned that this protein might also control the expression of enzymes involved in fatty acid re-esterification. In adipose tissue from FAT/CD36 KO mice we found that glycerol and fatty acid release were reduced and this was associated with reductions in ATGL. Decreases in lipolysis were paralleled by increases in the FFA to glycerol ratio and reductions in primary and fractional rates of fatty acid re-esterfication in cultured adipose tissue from FAT/CD36 KO mice. Reductions in re-esterfication were associated with decreases in the mRNA expression and protein content of PEPCK. To determine if reductions in lipolysis could lead to decreases in PEPCK mRNA expression we treated cultured mouse adipose tissue with the lipase inhibitor CAY10499 (2 μM) and found that this resulted in an ~ 50% reduction in PEPCK mRNA expression. Treatment with hexarelin (10 μM, 12 hours), a CD36 agonist, increased PEPCK mRNA expression independent of lipolysis. Collectively our results provide novel evidence that FAT/CD36 regulates PEPCK in adipose tissue and that this could be secondary to reductions in lipolysis.
Authors:
Zhongxiao Wan; Sarthak Matravadia; Graham P Holloway; David C Wright
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  -     ISSN:  1522-1563     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
University of Alberta.
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