| Regulated vectorial secretion of cholesteryl ester transfer protein (LTP-I) by the CaCo-2 model of human enterocyte epithelium. | |
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MedLine Citation:
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PMID: 2837464 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have investigated the human CaCo-2 enterocyte model for secretion of the plasma cholesteryl ester transfer protein, LTP-I. CaCo-2 cells secrete a cholesteryl ester transfer protein which possesses molecular identity with plasma LTP-I, demonstrated by anti-LTP-I immunoblot analysis and immunoinhibition of all cell-secreted cholesteryl ester transfer activity. When CaCo-2 are cultured on permeable membranes, cholesteryl ester transfer activity is detected only in the lower culture compartment. Thus, CaCo-2 vectorially sort and secrete LTP-I, as well as the intestinal apolipoproteins, from the basolateral cellular domain. Over a 24-h period, CaCo-2 secrete cholesteryl ester transfer activity in a time-dependent manner, at approximately twice the rate of HepG2. Furthermore, CaCo-2 enterocytes, but not HepG2 hepatocytes, regulate LTP-I secretion in response to fatty acid concentration in the culture medium. Based on these observations, we speculate that the intestine may be the principal regulated source of human plasma LTP-I. |
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Authors:
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R A Faust; J J Albers |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 263 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1988 Jun |
Date Detail:
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Created Date: 1988-07-27 Completed Date: 1988-07-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 8786-9 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Washington School of Medicine, Seattle 98104. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apolipoproteins
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secretion* Carcinoma, Hepatocellular / metabolism Carrier Proteins / secretion* Cell Line Cholesterol Ester Transfer Proteins Epithelium / metabolism Glycoproteins* Humans Intestines / metabolism Kinetics Liver Neoplasms / metabolism Models, Biological Oleic Acid Oleic Acids / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins; 0/CETP protein, human; 0/Carrier Proteins; 0/Cholesterol Ester Transfer Proteins; 0/Glycoproteins; 0/Oleic Acids; 112-80-1/Oleic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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