| Regulated proteolysis of a transcription factor complex is critical to cell cycle progression in Caulobacter crescentus. | |
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MedLine Citation:
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PMID: 23368090 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Cell cycle transitions are often triggered by the proteolysis of key regulatory proteins. In Caulobacter crescentus, the G1-S transition involves the degradation of an essential DNA-binding response regulator, CtrA, by the ClpXP protease. Here, we show that another critical cell cycle regulator, SciP, is also degraded during the G1-S transition, but by the Lon protease. SciP is a small protein that binds directly to CtrA and prevents it from activating target genes during G1. We demonstrate that SciP must be degraded during the G1-S transition so that cells can properly activate CtrA-dependent genes following DNA replication initiation and the reaccumulation of CtrA. These results indicate that like CtrA, SciP levels are tightly regulated during the Caulobacter cell cycle. In addition, we show that formation of a complex between CtrA and SciP at target promoters protects both proteins from their respective proteases. Degradation of either protein thus helps trigger the destruction of the other, facilitating a cooperative disassembly of the complex. Collectively, our results indicate that ClpXP and Lon each degrade a critical cell cycle regulator, helping to trigger the onset of S phase and prepare cells for the subsequent programs of gene expression critical to polar morphogenesis and cell division. |
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Authors:
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Kasia G Gora; Amber Cantin; Matt Wohlever; Kamal K Joshi; Barrett S Perchuk; Peter Chien; Michael T Laub |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-2-1 |
Journal Detail:
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Title: Molecular microbiology Volume: - ISSN: 1365-2958 ISO Abbreviation: Mol. Microbiol. Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-2-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2013 Blackwell Publishing Ltd. |
Affiliation:
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Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139; Howard Hughes Medical Institute, Cambridge, MA, 02139. |
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