Document Detail

Regulated lens regeneration from isolated pigmented epithelial cells of newt iris in culture in response to FGF2/4.
MedLine Citation:
PMID:  12076337     Owner:  NLM     Status:  MEDLINE    
When a lens is removed from the newt eye, a new lens is regenerated from the pigmented epithelial cells of the dorsal iris, whereas the ventral iris never shows such an ability. It is important to clarify the nature of signaling molecules which act directly on the iris cells to accomplish lens regeneration from the iris and also to gain insight into the mechanism of dorso-ventral difference of the regeneration potential. To examine the effects of exogenous factors, we established an in vitro culture of reaggregates made from dissociated pigmented epithelial cells of dorsal or ventral halves of newt iris. Foci of depigmented cells appeared within the cell reaggregates, regardless of their origins, when the cell reaggregates were cultured with FGF2 or FGF4. In contrast, only the depigmented cells in the dorsal iris cell reaggregates underwent extensive proliferation and developed a lens with the synthesis of lens-specific crystallins, recapitulating the normal lens regeneration. On the other hand, neither FGF8, FGF10, EGF, VEGF, nor IGF promoted lens development from iris cell reaggregates. Consistent with the FGF-specific action, FGFR-specific inhibitor SU5402 suppressed the lens development from the cultured cell reaggregates. These results demonstrated that FGF2 or FGF4 is essential for the in vitro lens regeneration from the pigmented cells of the dorsal iris. In addition, these findings indicated that unequal competence in the dorsal and ventral iris to FGF2/4 contributes to the difference in lens forming ability between them.
Toshinori Hayashi; Nobuhiko Mizuno; Katsushi Owaribe; Atsushi Kuroiwa; Mitsumasa Okamoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  70     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-06-21     Completed Date:  2002-12-23     Revised Date:  2003-12-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  101-8     Citation Subset:  IM    
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
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MeSH Terms
Cells, Cultured
Epithelial Cells / cytology,  drug effects*,  metabolism
Fibroblast Growth Factor 2 / pharmacology*
Fibroblast Growth Factors / pharmacology*
Lens, Crystalline / growth & development,  physiology*
Pigment Epithelium of Eye / cytology,  metabolism
Regeneration / physiology*
Salamandridae / anatomy & histology,  physiology*
Reg. No./Substance:
0/fibroblast growth factor 14; 103107-01-3/Fibroblast Growth Factor 2; 62031-54-3/Fibroblast Growth Factors

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