| Regulated expression of intrinsic factor-cobalamin receptor by rat visceral yolk sac and placental membranes. | |
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MedLine Citation:
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PMID: 8384000 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intrinsic factor-cobalamin receptor (IFCR) activity in visceral yolk sac and placental membranes is regulated during pregnancy in rats. While the IFCR activity declined in the visceral yolk sac membranes by 15-fold, it rose nearly 20-fold in the placental membranes from fourteen to nineteen days of gestation. The visceral yolk sac membranes revealed a 230 kDa protein that co-migrated with pure rat renal IFCR. This 230 kDa band was also identified as IFCR in both the membranes by immunoblotting with anti-serum to rat renal IFCR. Immunoprecipitation of 35S labeled proteins obtained from in vitro translation using visceral yolk sac mRNA from 14-day pregnant rats, yielded on SDS-PAGE a single band of 220 kDa, while those obtained from 19-day pregnant rats did not. The binding of intrinsic factor-cyano[57Co]cobalamin complex to the visceral yolk sac membranes was inhibited by preincubation of these membranes with anti-serum to rat IFCR but not with anti-serum to rat asialoglycoprotein receptor or mannose or mannan or N-acetylglucosamine. Based on these results, we suggest that the IFCR activity, protein expression and mRNA levels in fetal membranes are regulated during pregnancy and may play an important role in the maternal-fetal transfer of cobalamin. |
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Authors:
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K S Ramanujam; S Seetharam; B Seetharam |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1146 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1993 Mar |
Date Detail:
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Created Date: 1993-04-22 Completed Date: 1993-04-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 243-6 Citation Subset: IM |
Affiliation:
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Department of Medicine, Medical College of Wisconsin, Milwaukee 53226. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Extraembryonic Membranes / metabolism* Female Gene Expression Regulation Immune Sera Membrane Proteins / genetics*, isolation & purification Placenta / metabolism* RNA, Messenger / analysis Rats Receptors, Cell Surface / chemistry, genetics*, metabolism Receptors, Peptide* Vitamin B 12 / metabolism* Yolk Sac / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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NIDDK-26638/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immune Sera; 0/Membrane Proteins; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/Receptors, Peptide; 0/intrinsic factor receptor; 68-19-9/Vitamin B 12 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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