| Regulated expression of the interferon-beta gene in mice. | |
| | |
MedLine Citation:
|
PMID: 17637794 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A single plasmid regulated expression vector based upon a mifepristone-inducible two plasmid system, termed pBRES, has been constructed and tested in mice using murine interferon-b (mIFNb) as the transgene. The expression of mIFNb in the circulation was followed by measuring the systemic induction of IP-10, a validated biomarker for mIFNb in mice. Long-term, inducible expression of mIFNb was demonstrated following a single intramuscular (i.m.) injection of the pBRES mIFNb plasmid vector into the hind limb of mice. Induction of mIFNb expression was achieved by administration of the small molecule inducer, mifepristone (MFP). Plasmid DNA and mIFNb mRNA levels in the injected muscles correlated with mIFNb expression as monitored by IP-10 over a 3-month time period. Renewable transgene expression was achieved following repeat administration of the plasmid at 3 months following the first plasmid injection. A dose-dependent increase in expression was demonstrated by varying the amount of injected plasmid or the amount of the inducer administered to the mice. Finally, the pBRES plasmid expressing mIFNb under control of the inducer, MFP, was shown to be efficacious in a murine model of experimental allergic encephalomyelitis, supporting the feasibility of gene-based therapeutic approaches for treating diseases such as multiple sclerosis. |
| | |
Authors:
|
R N Harkins; P Szymanski; H Petry; A Brooks; H S Qian; C Schaefer; P J Kretschmer; A Orme; P Wang; G M Rubanyi; T W Hermiston |
Related Documents
:
|
23551434 - Epithelial-mesenchymal transition and clinicopathological correlation in craniopharyngi... 14625394 - Cell history determines the maintenance of transcriptional differences between left and... 11553794 - Delivery of the cre recombinase by a self-deleting lentiviral vector: efficient gene ta... 10601664 - The t3(b) gene promoter directs intestinal epithelial cell-specific expression in trans... 21770734 - Comparison of the patterns of milk ejection during repeated breast expression sessions ... 11408924 - Accumulation of extracellular matrix in the liver induces high metastatic potential of ... |
Publication Detail:
|
Type: Journal Article Date: 2007-07-19 |
Journal Detail:
|
Title: Gene therapy Volume: 15 ISSN: 1476-5462 ISO Abbreviation: Gene Ther. Publication Date: 2008 Jan |
Date Detail:
|
Created Date: 2007-12-18 Completed Date: 2008-04-11 Revised Date: 2010-07-29 |
Medline Journal Info:
|
Nlm Unique ID: 9421525 Medline TA: Gene Ther Country: England |
Other Details:
|
Languages: eng Pagination: 1-11 Citation Subset: IM |
Affiliation:
|
Department of Gene Technologies, Berlex Biosciences, Richmond, CA, USA. rick.harkins@bayer.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Markers / blood Chemokine CXCL10 / analysis Disease Progression Encephalomyelitis, Autoimmune, Experimental / therapy* Female Gene Expression Regulation* Gene Therapy / methods* Genetic Vectors / administration & dosage* Injections, Intramuscular Interferon-beta / blood, genetics* Mice Mice, Inbred Strains Mifepristone / administration & dosage Multiple Sclerosis / therapy Plasmids / administration & dosage*, analysis RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction Transgenes |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 0/Chemokine CXCL10; 0/RNA, Messenger; 77238-31-4/Interferon-beta; 84371-65-3/Mifepristone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Home-measured blood pressure is more strongly associated with electrocardiographic left ventricular ...
Next Document: Galactosylated chitosan-graft-polyethylenimine as a gene carrier for hepatocyte targeting.