Document Detail


Regulated expression of angiotensin II (AT2) binding sites in R3T3 cells.
MedLine Citation:
PMID:  8469774     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
R3T3 cells are a fibroblast cell line found to selectively express the AT2 subtype of angiotensin II binding sites. We have previously shown that in these cells, the AT2 sites do not appear to be coupled to G-proteins, do not modulate any of the common second messenger pathways associated with activation of angiotensin II receptors, and do not internalize bound ligand. Here we report that expression of AT2 sites in these cells are subject to modulation by a variety of conditions. In actively growing cells the expression of AT2 sites is very low, while in confluent, quiescent cells, the expression of AT2 sites is markedly increased. Addition of serum, or growth factors, to quiescent cells causes a rapid decrease in the number of cell-surface AT2 sites. Further, incubation of cells with ligands that bind to AT2 sites causes a marked increase in the number of these sites in a time and dose dependent manner indicating homologous up-regulation of expression. These results indicate that expression of AT2 sites in R3T3 cells is under sensitive and rapid control and further indicate that these cells may be an excellent model for studying the physiological regulation of expression of these sites.
Authors:
D T Dudley; R M Summerfelt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Regulatory peptides     Volume:  44     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-05-11     Completed Date:  1993-05-11     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  199-206     Citation Subset:  IM    
Affiliation:
Department of Signal Transduction, Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, Ann Arbor, MI 48105.
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MeSH Terms
Descriptor/Qualifier:
1-Sarcosine-8-Isoleucine Angiotensin II / pharmacology
3T3 Cells
Angiotensin II / metabolism*,  pharmacology
Animals
Biphenyl Compounds / pharmacology
Bombesin / pharmacology
Cell Division
Fibroblast Growth Factor 2 / pharmacology
Imidazoles / pharmacology
Losartan
Mice
Mitogens / pharmacology
Pyridines / pharmacology
Receptors, Angiotensin / antagonists & inhibitors,  metabolism*
Saralasin / pharmacology
Tetrazoles / pharmacology
Up-Regulation
Chemical
Reg. No./Substance:
0/Biphenyl Compounds; 0/Imidazoles; 0/Mitogens; 0/Pyridines; 0/Receptors, Angiotensin; 0/Tetrazoles; 103107-01-3/Fibroblast Growth Factor 2; 11128-99-7/Angiotensin II; 114798-26-4/Losartan; 130663-39-7/PD 123319; 31362-50-2/Bombesin; 34273-10-4/Saralasin; 9088-01-1/1-Sarcosine-8-Isoleucine Angiotensin II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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