Document Detail

Regrowth and radiation sensitivity of quiescent cells isolated from EMT6/Ro-fed plateau monolayers.
MedLine Citation:
PMID:  3971359     Owner:  NLM     Status:  MEDLINE    
A quiescent [denoted as Q(G0/G1)] subpopulation was isolated from EMT6/Ro-fed plateau monolayers by centrifugal elutriation. The median Coulter volume of these cells was significantly smaller than that of the original population from which they were elutriated. Using two-step acridine orange staining and dual parameter flow cytometric analysis, over 95% of quiescent cells were found to have G1 DNA content, and 80% of the cells had a decreased RNA content as compared to rapidly proliferating exponential G1 cells. After labeling for 24 hr (two doubling times) with [3H]thymidine, less than 2% of the quiescent cells incorporated [3H]thymidine as measured by autoradiography. The colony-forming efficiency of these cells was not significantly different from that of exponential cells. When such Q(G0/G1) cells were replated in fresh medium at a lower density, there was a lag time of 30 hr before any increase in cell number was detected, after which the cell-doubling rate matched that of exponential culture. Results obtained from the radiation dose-response curves showed that quiescent (G0/G1) cells were more radiosensitive than exponential G1 or unseparated fed plateau cells.
C K Luk; P C Keng; R M Sutherland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  45     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-03-27     Completed Date:  1985-03-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1020-5     Citation Subset:  IM    
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MeSH Terms
Cell Cycle
Cell Separation
Cell Survival / radiation effects*
Cells, Cultured
DNA, Neoplasm / analysis
Flow Cytometry
Mammary Neoplasms, Experimental / pathology*
RNA, Neoplasm / analysis
Grant Support
Reg. No./Substance:
0/DNA, Neoplasm; 0/RNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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