Document Detail


Regionally specific and genome-wide analyses conclusively demonstrate the absence of CpG methylation in human mitochondrial DNA.
MedLine Citation:
PMID:  23671186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although CpG methylation clearly distributes genome-wide in vertebrate nuclear DNA, the state of methylation in the vertebrate mitochondrial genome has been unclear. Several recent reports using immunoprecipitation, mass spectrometry, and enzyme-linked immunosorbent assay methods concluded that human mitochondrial DNA (mtDNA) has much more than the 2 to 5% CpG methylation previously estimated. However, these methods do not provide information as to the sites or frequency of methylation at each CpG site. Here, we have used the more definitive bisulfite genomic sequencing method to examine CpG methylation in HCT116 human cells and primary human cells to independently answer these two questions. We found no evidence of CpG methylation at a biologically significant level in these regions of the human mitochondrial genome. Furthermore, unbiased next-generation sequencing of sodium bisulfite treated total DNA from HCT116 cells and analysis of genome-wide sodium bisulfite sequencing data sets from several other DNA sources confirmed this absence of CpG methylation in mtDNA. Based on our findings using regionally specific and genome-wide approaches with multiple human cell sources, we can definitively conclude that CpG methylation is absent in mtDNA. It is highly unlikely that CpG methylation plays any role in direct control of mitochondrial function.
Authors:
Elizabeth E Hong; Cindy Y Okitsu; Andrew D Smith; Chih-Lin Hsieh
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-05-13
Journal Detail:
Title:  Molecular and cellular biology     Volume:  33     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-24     Completed Date:  2013-08-23     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2683-90     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Human / genetics
CpG Islands*
DNA Methylation*
DNA, Mitochondrial / genetics*
Genes, Mitochondrial*
Genome, Human
HCT116 Cells
High-Throughput Nucleotide Sequencing
Humans
Sequence Analysis, DNA
Grant Support
ID/Acronym/Agency:
R01HG005238/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Mitochondrial
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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