| Regional structure-function relationships in mouse aortic valve tissue. | |
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MedLine Citation:
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PMID: 20863504 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Site-specific biomechanical properties of the aortic valve play an important role in native valve function, and alterations in these properties may reflect mechanisms of degeneration and disease. Small animals such as targeted mutagenesis mice provide a powerful approach to model human valve disease pathogenesis; however, physical mechanical testing in small animals is limited by valve tissue size. Aortic valves are comprised of highly organized extracellular matrix compartmentalized in cusp and annulus regions, which have different functions. The objective of this study was to measure regional mechanical properties of mouse aortic valve tissue using a modified micropipette aspiration technique. Aortic valves were isolated from juvenile, adult and aged adult C57BL/6 wild type mice. Tissue tensile stiffness was determined for annulus and cusp regions using a half-space punch model. Stiffness for the annulus region was significantly higher compared to the cusp region at all stages. Further, aged adult valve tissue had decreased stiffness in both the cusp and annulus. Quantitative histochemical analysis revealed a collagen-rich annulus and a proteoglycan-rich cusp at all stages. In aged adult valves, there was proteoglycan infiltration of the annulus hinge, consistent with the observed mechanical differences over time. These findings indicate that valve tissue biomechanical properties vary in wild type mice in a region-specific and age-related manner. The micropipette aspiration technique provides a promising approach for studies of valve structure and function in small animal models, such as transgenic mouse models of valve disease. |
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Authors:
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Varun K Krishnamurthy; Farshid Guilak; Daria A Narmoneva; Robert B Hinton |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-09-21 |
Journal Detail:
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Title: Journal of biomechanics Volume: 44 ISSN: 1873-2380 ISO Abbreviation: J Biomech Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-17 Completed Date: 2011-04-12 Revised Date: 2012-01-06 |
Medline Journal Info:
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Nlm Unique ID: 0157375 Medline TA: J Biomech Country: United States |
Other Details:
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Languages: eng Pagination: 77-83 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Biomedical Engineering, University of Cincinnati, Cincinnati, OH, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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pathology,
physiology Animals Aortic Valve / anatomy & histology*, physiology* Biomechanics Collagen / physiology Elasticity Extracellular Matrix / physiology Heart Valve Diseases / etiology, pathology, physiopathology Histological Techniques / instrumentation, methods Humans Mice Mice, Inbred C57BL Models, Animal Models, Cardiovascular Proteoglycans / physiology Tensile Strength / physiology Viscosity |
| Grant Support | |
ID/Acronym/Agency:
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K23 HL085122-05/HL/NHLBI NIH HHS; K23HL085122/HL/NHLBI NIH HHS; R01AR15768/AR/NIAMS NIH HHS; R21DK078814/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proteoglycans; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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