| Regional recruitment of rat diaphragmatic lymphatics in response to increased pleural or peritoneal fluid load. | |
| | |
MedLine Citation:
|
PMID: 17218349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The specific role of the diaphragmatic tendinous and muscular tissues in sustaining lymph formation and propulsion in the diaphragm was studied in 24 anaesthetized spontaneously breathing supine rats. Three experimental protocols were used: (a) control; (b) peritoneal ascitis, induced through an intraperitoneal injection of 100 ml kg(-1) of iso-oncotic saline; and (c) pleural effusion, induced through an intrapleural injection of 6.6 ml kg(-1) saline solution. A group of animals (n = 12) was instrumented to measure the hydraulic transdiaphragmatic pressure gradient between the pleural and peritoneal cavities in the three protocols. In the other group (n = 12), the injected iso-oncotic saline was enriched with 2% fluorescent dextrans (molecular mass = 70 kDa); at 30 min from the injections these animals were suppressed and their diaphragm excised and processed for confocal microscopy analysis. In control conditions, in spite of a favourable peritoneal-to-pleural pressure gradient, the majority of the tracer absorbed into the diaphragmatic lymphatic system converges towards the deeper collecting lymphatic ducts. This suggests that diaphragmatic lymph formation mostly depends upon pressure gradients developing between the serosal cavities and the lymphatic vessel lumen. In addition, the tracer distributes to lymph vessels located in the muscular diaphragmatic tissue, suggesting that active muscle contraction, rather than passive tendon stretch, more efficiently enhances local diaphragmatic lymph flow. Vice versa, a prevailing recruitment of the lymphatics of the tendinous diaphragmatic regions was observed in peritoneal ascitis and pleural effusion, suggesting a functional adaptation of the diaphragmatic network to increased draining requirements. |
| | |
Authors:
|
Andrea Moriondo; Annalisa Grimaldi; Laura Sciacca; Maria Luisa Guidali; Cristiana Marcozzi; Daniela Negrini |
Related Documents
:
|
7234019 - Surgical pathophysiology of the lymphatic system. 1144449 - Effects of ischemia on forelimb weight and lymph protein concentration. 1668019 - Pulmonary edema: ischemia reperfusion endothelial injury and its reversal by c-amp. 9212719 - A technique for chronically recording the ekg of the lymph hearts in the toad bufo aren... 6430959 - Effects of asphyxia on lung fluid balance in baby lambs. 10444399 - Tissue factor activity is increased in human endothelial cells cultured under elevated ... 2973309 - 2-mercaptopropionylglycine improves aortic flow after reoxygenation in working rat hearts. 16221099 - Renal plasma flow, filtration fraction and microalbuminuria in hypertensive patients: e... 3342209 - An analysis of uterine activity in spontaneous labour using a microcomputer. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-11 |
Journal Detail:
|
Title: The Journal of physiology Volume: 579 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2007 Mar |
Date Detail:
|
Created Date: 2007-03-16 Completed Date: 2007-05-16 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
|
Languages: eng Pagination: 835-47 Citation Subset: IM |
Affiliation:
|
Dipartimento di Scienze Biomediche Sperimentali e Cliniche, Università degli Studi dell'Insubria, Via J.H. Dunant 5, 21100 Varese, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acute Disease Animals Ascites / metabolism, physiopathology* Body Fluids / metabolism Diaphragm / physiology* Fluorescent Dyes / pharmacokinetics Hydrostatic Pressure Lymphatic System / physiology* Male Peritoneal Cavity / physiology* Pleural Cavity / physiology* Pleural Effusion / metabolism, physiopathology* Rats Rats, Wistar Solutions / pharmacokinetics |
| Chemical | |
Reg. No./Substance:
|
0/Fluorescent Dyes; 0/Solutions |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular basis of the T- and L-type Ca2+ currents in canine Purkinje fibres.
Next Document: The kinetics of inhibition of rat recombinant heteromeric alpha1beta glycine receptors by the low-af...