| Regional mRNA expression of key gluconeogenic enzymes in the liver of dairy cows. | |
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MedLine Citation:
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PMID: 19906140 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Liver tissue was collected from eight random dairy cows at a slaughterhouse to test if gene expression of pyruvate carboxylase (PC), mitochondrial phosphoenolpyruvate carboxykinase (PEPCKm) and cytosolic phosphoenolpyruvate carboxykinase (PEPCKc) is different at different locations in the liver. Obtained liver samples were analysed for mRNA expression levels of PC, PEPCKc and PEPCKm and subjected to the MIXED procedure of SAS to test for the sampled locations with cow liver as repeated subject. Additionally, the general linear model procedure (GLM) for analysis of variance was applied to test for significant differences for mRNA abundance of PEPCKm, PEPCKc and bPC between the livers. In conclusion, this study demonstrated that mRNA abundance of PC, PEPCKc and PEPCKm is not different between locations in the liver but may differ between individual cows. |
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Authors:
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H A Van Dorland; R M Bruckmaier |
Publication Detail:
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Type: Journal Article Date: 2009-11-11 |
Journal Detail:
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Title: Journal of animal physiology and animal nutrition Volume: 94 ISSN: 1439-0396 ISO Abbreviation: J Anim Physiol Anim Nutr (Berl) Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-23 Completed Date: 2010-11-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101126979 Medline TA: J Anim Physiol Anim Nutr (Berl) Country: Germany |
Other Details:
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Languages: eng Pagination: 505-8 Citation Subset: IM |
Affiliation:
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Veterinary Physiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland. anette.vandorland@physio.unibe.ch |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed Animal Nutritional Physiological Phenomena / physiology* Animals Cattle / metabolism* Dairying Female Gene Expression Regulation, Enzymologic Gluconeogenesis / physiology* Liver / enzymology* Phosphoenolpyruvate Carboxykinase (ATP) / metabolism Phosphoenolpyruvate Carboxylase / metabolism Pyruvate Carboxylase / metabolism RNA, Messenger / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; EC 4.1.1.31/Phosphoenolpyruvate Carboxylase; EC 4.1.1.49/Phosphoenolpyruvate Carboxykinase (ATP); EC 6.4.1.1/Pyruvate Carboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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