Document Detail


Regional heterogeneity in astrocyte responses following contusive spinal cord injury in mice.
MedLine Citation:
PMID:  20151365     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Astrocytes and their precursors respond to spinal cord injury (SCI) by proliferating, migrating, and altering phenotype. This contributes to glial scar formation at the lesion border and gliosis in spared gray and white matter. The present study was undertaken to evaluate astrocyte changes over time and determine when and where interventions might be targeted to alter the astrocyte response. Bromodeoxyuridine (BrdU) was administered to mice 3 days after SCI, and cells expressing BrdU and the astrocyte marker, glial fibrillary acidic protein (GFAP), were counted at 3, 7, and 49 days post-injury (DPI). BrdU-labeled cells accumulated at the lesion border by 7 DPI and approximately half of these expressed GFAP. In spared white matter, the total number of BrdU+ cells decreased, while the percentage of BrdU+ cells expressing GFAP increased at 49 DPI. Phenotypic changes were examined using the progenitor marker nestin, the radial glial marker, brain lipid binding protein (BLBP), and GFAP. Nestin was upregulated by 3 DPI and declined between 7 and 49 DPI in all regions, and GFAP increased and remained above naïve levels at all timepoints. BLBP increased early and remained high along the lesion border and spared white matter, but was expressed transiently by cells lining the central canal and in a unique population of small cells found within the lesion and in gray matter rostral and caudal to the border. The results demonstrate that the astrocyte response to SCI is regionally heterogeneous, and suggests astrocyte populations that could be targeted by interventions.
Authors:
Robin E White; Dana M McTigue; Lyn B Jakeman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  518     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-06-01     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1370-90     Citation Subset:  IM    
Copyright Information:
(c) 2009 Wiley-Liss, Inc.
Affiliation:
Neuroscience Graduate Studies Program, Ohio State University, Columbus, Ohio 43210, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / physiology*
Bromodeoxyuridine
Cell Count
Cell Proliferation
Fatty Acid-Binding Proteins / metabolism
Female
Fluorescent Antibody Technique
Glial Fibrillary Acidic Protein / metabolism
Immunohistochemistry
Intermediate Filament Proteins / metabolism
Mice
Mice, Inbred C57BL
Nerve Fibers, Myelinated / physiology
Nerve Fibers, Unmyelinated / physiology
Nerve Tissue Proteins / metabolism
Phenotype
Spinal Cord Injuries / physiopathology*
Time Factors
Grant Support
ID/Acronym/Agency:
P30-NS045748/NS/NINDS NIH HHS; R01 NS043246/NS/NINDS NIH HHS; R01 NS043246/NS/NINDS NIH HHS; R01 NS043246-05/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Fabp7 protein, mouse; 0/Fatty Acid-Binding Proteins; 0/Glial Fibrillary Acidic Protein; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/nestin; 59-14-3/Bromodeoxyuridine
Comments/Corrections

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