| Regional heterogeneity in astrocyte responses following contusive spinal cord injury in mice. | |
| | |
MedLine Citation:
|
PMID: 20151365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Astrocytes and their precursors respond to spinal cord injury (SCI) by proliferating, migrating, and altering phenotype. This contributes to glial scar formation at the lesion border and gliosis in spared gray and white matter. The present study was undertaken to evaluate astrocyte changes over time and determine when and where interventions might be targeted to alter the astrocyte response. Bromodeoxyuridine (BrdU) was administered to mice 3 days after SCI, and cells expressing BrdU and the astrocyte marker, glial fibrillary acidic protein (GFAP), were counted at 3, 7, and 49 days post-injury (DPI). BrdU-labeled cells accumulated at the lesion border by 7 DPI and approximately half of these expressed GFAP. In spared white matter, the total number of BrdU+ cells decreased, while the percentage of BrdU+ cells expressing GFAP increased at 49 DPI. Phenotypic changes were examined using the progenitor marker nestin, the radial glial marker, brain lipid binding protein (BLBP), and GFAP. Nestin was upregulated by 3 DPI and declined between 7 and 49 DPI in all regions, and GFAP increased and remained above naïve levels at all timepoints. BLBP increased early and remained high along the lesion border and spared white matter, but was expressed transiently by cells lining the central canal and in a unique population of small cells found within the lesion and in gray matter rostral and caudal to the border. The results demonstrate that the astrocyte response to SCI is regionally heterogeneous, and suggests astrocyte populations that could be targeted by interventions. |
| | |
Authors:
|
Robin E White; Dana M McTigue; Lyn B Jakeman |
Related Documents
:
|
18683635 - The development of the drosophila larval brain. 1983315 - The origins of cell diversity in the insect central nervous system. 1525515 - Neuronal specification. 9154155 - Cellular distribution of branched-chain amino acid aminotransferase isoenzymes among ra... 23132795 - Al(2) o(3) nanoparticles induce mitochondria-mediated cell death and upregulate the exp... 19066725 - Synthesis of novel 6-fluoro-3-(4-piperidinyl)-1,2-benzisoxazole derivatives as antiprol... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: The Journal of comparative neurology Volume: 518 ISSN: 1096-9861 ISO Abbreviation: J. Comp. Neurol. Publication Date: 2010 Apr |
Date Detail:
|
Created Date: 2010-02-22 Completed Date: 2010-06-01 Revised Date: 2012-10-09 |
Medline Journal Info:
|
Nlm Unique ID: 0406041 Medline TA: J Comp Neurol Country: United States |
Other Details:
|
Languages: eng Pagination: 1370-90 Citation Subset: IM |
Copyright Information:
|
(c) 2009 Wiley-Liss, Inc. |
Affiliation:
|
Neuroscience Graduate Studies Program, Ohio State University, Columbus, Ohio 43210, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Astrocytes / physiology* Bromodeoxyuridine Cell Count Cell Proliferation Fatty Acid-Binding Proteins / metabolism Female Fluorescent Antibody Technique Glial Fibrillary Acidic Protein / metabolism Immunohistochemistry Intermediate Filament Proteins / metabolism Mice Mice, Inbred C57BL Nerve Fibers, Myelinated / physiology Nerve Fibers, Unmyelinated / physiology Nerve Tissue Proteins / metabolism Phenotype Spinal Cord Injuries / physiopathology* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
|
P30-NS045748/NS/NINDS NIH HHS; R01 NS043246/NS/NINDS NIH HHS; R01 NS043246/NS/NINDS NIH HHS; R01 NS043246-05/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Fabp7 protein, mouse; 0/Fatty Acid-Binding Proteins; 0/Glial Fibrillary Acidic Protein; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/nestin; 59-14-3/Bromodeoxyuridine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Actions of motor neurons and leg muscles in jumping by planthopper insects (hemiptera, issidae).
Next Document: Classification of eating disturbance in children and adolescents: proposed changes for the DSM-V.