Document Detail

Regional distribution of the GABAA/benzodiazepine receptor (alpha subunit) mRNA in rat brain.
MedLine Citation:
PMID:  2844998     Owner:  NLM     Status:  MEDLINE    
A human cDNA clone containing the 5' coding region of the GABAA/benzodiazepine receptor alpha subunit was used to quantify and visualize receptor mRNA in various regions of the rat brain. Using a [32P]CTP-labelled antisense RNA probe (860 bases) prepared from the alpha subunit cDNA, multiple mRNA species were detected in Northern blots using total and poly A rat brain RNA. In all brain regions, mRNAs of 4.4 and 4.8 kb were observed, and an additional mRNA of 3.0 kb was detected in the cerebellum and hippocampus. The level of GABAA/benzodiazepine receptor mRNA was highest in the cerebellum followed by the thalamus = frontal cortex = hippocampus = parietal cortex = hypothalamus much greater than pons = striatum = medulla. In situ hybridization revealed high levels of alpha subunit mRNA in cerebellar gray matter, olfactory bulb, thalamus, hippocampus/dentate gyrus, and the arcuate nucleus of the hypothalamus. These data suggest the presence of multiple GABAA/benzodiazepine receptor alpha subunit mRNAs in rat brain and demonstrate the feasibility of studying the expression of genes encoding the GABAA/benzodiazepine receptor after pharmacological and/or environmental manipulation.
P Montpied; B M Martin; S L Cottingham; B K Stubblefield; E I Ginns; S M Paul
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  51     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1988-11-22     Completed Date:  1988-11-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1651-4     Citation Subset:  IM    
Section on Molecular Pharmacology, NIMH, Bethesda, Maryland 20892.
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MeSH Terms
Blotting, Northern
Brain Chemistry*
Cerebellum / analysis
Cerebral Cortex / analysis
Hippocampus / analysis
Hypothalamus / analysis
Nucleic Acid Hybridization
RNA Probes
RNA, Messenger / analysis*
Rats, Inbred Strains
Receptors, GABA-A / genetics*
Thalamus / analysis
Tissue Distribution
Reg. No./Substance:
0/RNA Probes; 0/RNA, Messenger; 0/Receptors, GABA-A

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