| Regional differences in lectin binding patterns of vestibular hair cells. | |
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MedLine Citation:
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PMID: 8458748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Surface glycoconjugates of hair cells and supporting cells in the vestibular endorgans of the bullfrog were identified using biotinylated lectins with different carbohydrate specificities. Lectin binding in hair cells was consistent with the presence of glucose and mannose (CON A), galactose (RCA-I), N-acetylglucosamine (WGA), N-acetylgalactosamine (VVA), but not fucose (UEA-I) residues. Hair cells in the bullfrog sacculus, unlike those in the utriculus and semicircular canals, did not strain for N-acetylglucosamine (WGA) or N-acetylgalactosamine (VVA). By contrast, WGA and, to a lesser extent, VVA, differentially stained utricular and semicircular canal hair cells, labeling hair cells located in peripheral, but not central, regions. In mammals, WGA uniformly labeled Type I hair cells while labeling, as in the bullfrog, Type II hair cells only in peripheral regions. These regional variations were retained after enzymatic digestion. We conclude that vestibular hair cells differ in their surface glycoconjugates and that differences in lectin binding patterns can be used to identify hair cell types and to infer the epithelial origin of isolated vestibular hair cells. |
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Authors:
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R A Baird; N R Schuff; J Bancroft |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Hearing research Volume: 65 ISSN: 0378-5955 ISO Abbreviation: Hear. Res. Publication Date: 1993 Feb |
Date Detail:
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Created Date: 1993-04-23 Completed Date: 1993-04-23 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7900445 Medline TA: Hear Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 151-63 Citation Subset: IM; S |
Affiliation:
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R.S. Dow Neurological Sciences Institute, Good Samaritan Hospital and Medical Center, Portland, Oregon 97209. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylgalactosamine
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metabolism Acetylglucosamine / metabolism Animals Binding Sites Carbohydrate Sequence Fucose / metabolism Galactose / metabolism Glucose / metabolism Glycoconjugates / analysis*, metabolism Guinea Pigs Hair Cells, Auditory / chemistry, metabolism* Lectins / metabolism* Mannose / metabolism Molecular Sequence Data Rana catesbeiana Saccule and Utricle / chemistry, metabolism Vestibule, Labyrinth / chemistry, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DC-00355/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glycoconjugates; 0/Lectins; 26566-61-0/Galactose; 31022-50-1/Acetylgalactosamine; 31103-86-3/Mannose; 3713-31-3/Fucose; 50-99-7/Glucose; 7512-17-6/Acetylglucosamine |
| Investigator | |
Investigator/Affiliation:
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R A Baird / RS Dow Neurological Sci Inst, Portland, OR |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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