Document Detail


Regional differences in lectin binding patterns of vestibular hair cells.
MedLine Citation:
PMID:  8458748     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Surface glycoconjugates of hair cells and supporting cells in the vestibular endorgans of the bullfrog were identified using biotinylated lectins with different carbohydrate specificities. Lectin binding in hair cells was consistent with the presence of glucose and mannose (CON A), galactose (RCA-I), N-acetylglucosamine (WGA), N-acetylgalactosamine (VVA), but not fucose (UEA-I) residues. Hair cells in the bullfrog sacculus, unlike those in the utriculus and semicircular canals, did not strain for N-acetylglucosamine (WGA) or N-acetylgalactosamine (VVA). By contrast, WGA and, to a lesser extent, VVA, differentially stained utricular and semicircular canal hair cells, labeling hair cells located in peripheral, but not central, regions. In mammals, WGA uniformly labeled Type I hair cells while labeling, as in the bullfrog, Type II hair cells only in peripheral regions. These regional variations were retained after enzymatic digestion. We conclude that vestibular hair cells differ in their surface glycoconjugates and that differences in lectin binding patterns can be used to identify hair cell types and to infer the epithelial origin of isolated vestibular hair cells.
Authors:
R A Baird; N R Schuff; J Bancroft
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hearing research     Volume:  65     ISSN:  0378-5955     ISO Abbreviation:  Hear. Res.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-04-23     Completed Date:  1993-04-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7900445     Medline TA:  Hear Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  151-63     Citation Subset:  IM; S    
Affiliation:
R.S. Dow Neurological Sciences Institute, Good Samaritan Hospital and Medical Center, Portland, Oregon 97209.
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MeSH Terms
Descriptor/Qualifier:
Acetylgalactosamine / metabolism
Acetylglucosamine / metabolism
Animals
Binding Sites
Carbohydrate Sequence
Fucose / metabolism
Galactose / metabolism
Glucose / metabolism
Glycoconjugates / analysis*,  metabolism
Guinea Pigs
Hair Cells, Auditory / chemistry,  metabolism*
Lectins / metabolism*
Mannose / metabolism
Molecular Sequence Data
Rana catesbeiana
Saccule and Utricle / chemistry,  metabolism
Vestibule, Labyrinth / chemistry,  metabolism*
Grant Support
ID/Acronym/Agency:
DC-00355/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/Glycoconjugates; 0/Lectins; 26566-61-0/Galactose; 31022-50-1/Acetylgalactosamine; 31103-86-3/Mannose; 3713-31-3/Fucose; 50-99-7/Glucose; 7512-17-6/Acetylglucosamine
Investigator
Investigator/Affiliation:
R A Baird / RS Dow Neurological Sci Inst, Portland, OR

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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