Document Detail


Regional differences in colonic mucosa-associated microbiota determine the physiological expression of host heat shock proteins.
MedLine Citation:
PMID:  20864653     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytoprotective heat shock proteins (Hsps) are critical for intestinal homeostasis and are known to be decreased in inflammatory bowel diseases. Signals responsible for maintenance of Hsp expression are incompletely understood. In this study, we find that Hsp25/27 and Hsp70 protein expressions are differentially regulated along the longitudinal length of the large intestine, being highest in the proximal colon and decreasing to the distal colon. This longitudinal gradient was similar in both conventionally colonized mouse colon as well as biopsies of human proximal and distal colon but was abolished in the colon of germ-free mice, suggesting a role of intestinal microbiota in the Hsp regional expression. Correspondingly, analysis of 16S ribosomal RNA genes of bacteria from each colonic segment indicated increased bacterial richness and diversity in the proximal colon. The mechanism of regulation is transcriptional, as Hsp70 mRNA followed a similar pattern to Hsp70 protein expression. Lysates of mucosa-associated bacteria from the proximal colon stimulated greater Hsp25 and Hsp70 mRNA transcription and subsequent protein expression in intestinal epithelial cells than did lysates from distal colon. In addition, transrectal administration of cecal contents stimulated Hsp25 and Hsp70 expression in the distal colon. Thus host-microbial interactions resulting in differential Hsp expression may have significant implications for the maintenance of intestinal homeostasis and possibly for development of inflammatory diseases of the bowel.
Authors:
Shien Hu; Yunwei Wang; Lev Lichtenstein; Yun Tao; Mark W Musch; Bana Jabri; Dionysios Antonopoulos; Erika C Claud; Eugene B Chang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-09-23
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  299     ISSN:  1522-1547     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-02     Completed Date:  2011-01-18     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G1266-75     Citation Subset:  IM    
Affiliation:
Dept. of Medicine, The Univ. of Chicago, IL 60637, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biopsy
Cecum / microbiology
Cells, Cultured
Colon / anatomy & histology,  cytology,  metabolism,  microbiology*
Gene Expression Regulation / physiology*
Germ-Free Life
Heat-Shock Proteins / genetics,  metabolism*
Humans
Intestinal Mucosa / microbiology*
Jejunum / metabolism,  microbiology
Mice
Mice, Inbred C57BL
Phylogeny
RNA, Messenger / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
DK-42086/DK/NIDDK NIH HHS; DK-47722/DK/NIDDK NIH HHS; DK083993/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Heat-Shock Proteins; 0/RNA, Messenger
Comments/Corrections

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