| Regional differences in cellular mechanisms of adipose tissue gain with overfeeding. | |
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MedLine Citation:
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PMID: 20921416 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Body fat distribution is an important predictor of the metabolic consequences of obesity, but the cellular mechanisms regulating regional fat accumulation are unknown. We assessed the changes in adipocyte size (photomicrographs) and number in response to overfeeding in upper- and lower-body s.c. fat depots of 28 healthy, normal weight adults (15 men) age 29 ± 2 y. We analyzed how these changes relate to regional fat gain (dual energy X-ray absorptiometry and computed tomography) and baseline preadipocyte proliferation, differentiation [peroxisome proliferator-activated receptor-γ2 (PPARγ2) and CCAAT/enhancer binding protein-α (C/EBPα) mRNA]), and apoptotic response to TNF-α. Fat mass increased by 1.9 ± 0.2 kg in the upper body and 1.6 ± 0.1 kg in the lower body. Average abdominal s.c. adipocyte size increased by 0.16 ± 0.06 μg lipid per cell and correlated with relative upper-body fat gain (r = 0.74, P < 0.0001). However, lower-body fat responded to overfeeding by fat-cell hyperplasia, with adipocyte number increasing by 2.6 ± 0.9 × 10(9) cells (P < 0.01). We found no depot-differences in preadipocyte replication or apoptosis that would explain lower-body adipocyte hyperplasia and abdominal s.c. adipocyte hypertrophy. However, baseline PPARγ2 and C/EBPα mRNA were higher in abdominal than femoral s.c. preadipocytes (P < 0.005 and P < 0.03, respectively), consistent with the ability of abdominal s.c. adipocytes to achieve a larger size. Inherent differences in preadipocyte cell dynamics may contribute to the distinct responses of different fat depots to overfeeding, and fat-cell number increases in certain depots in adults after only 8 wk of increased food intake. |
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Authors:
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Yourka D Tchoukalova; Susanne B Votruba; Tamara Tchkonia; Nino Giorgadze; James L Kirkland; Michael D Jensen |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't Date: 2010-10-04 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-20 Completed Date: 2010-11-22 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 18226-31 Citation Subset: IM |
Affiliation:
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Endocrine Research Unit, Mayo Clinic, Rochester, MN 55905, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorptiometry, Photon Adipocytes / metabolism Adipose Tissue / cytology, metabolism* Adult Body Composition CCAAT-Enhancer-Binding Protein-alpha / genetics, metabolism Energy Intake* Female Humans Male PPAR gamma / genetics, metabolism Tomography, X-Ray Computed Tumor Necrosis Factor-alpha / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AG13925/AG/NIA NIH HHS; DK45343/DK/NIDDK NIH HHS; DK50456/DK/NIDDK NIH HHS; R01 AG013925-13/AG/NIA NIH HHS; R01 DK045343-20/DK/NIDDK NIH HHS; RR00585/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CCAAT-Enhancer-Binding Protein-alpha; 0/PPAR gamma; 0/Tumor Necrosis Factor-alpha |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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