Document Detail


Regional differences in BMP-dependence of dorsoventral patterning in the leech Helobdella.
MedLine Citation:
PMID:  22641012     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the leech Helobdella, the ectoderm exhibits a high degree of morphological homonomy between body segments, but pattern elements in lateral ectoderm arise via distinct cell lineages in the segments of the rostral and midbody regions. In each of the four rostral segments, a complete set of ventrolateral (O fate) and dorsolateral (P fate) ectodermal pattern elements arises from a single founder cell, op. In the 28 midbody and caudal segments, however, there are two initially indeterminate o/p founder cells; the more dorsal of these is induced to adopt the P fate by BMP5-8 emanating from the dorsalmost ectoderm, while the more ventral cell assumes the O fate. Previous work has suggested that the dorsoventral patterning of O and P fates differs in the rostral region, but the role of BMP signaling in those segments has not been investigated. We show here that suppression of dorsal BMP5-8 signaling (which effects a P-to-O fate change in the midbody) has no effect on the patterning of O and P fates in the rostral region. Furthermore, ectopic expression of BMP5-8 in the ventral ectoderm (which induces an O-to-P fate change in the midbody) has no effect in the rostral region. Finally, expression of a dominant-negative BMP receptor (which induces a P-to-O fate change in the midbody) fails to affect O/P patterning in the rostral region. Thus, the rostral segments appear to use some mechanism other than BMP signaling to pattern O and P cell fates along the dorsoventral axis. From a mechanistic standpoint, the OP lineage of the rostral segments and the O-P equivalence group of the midbody and caudal segments constitute distinct developmental modules that rely to differing degrees on positional cues from surrounding ectoderm in order to specify homonomous cell fates.
Authors:
Dian-Han Kuo; Marty Shankland; David A Weisblat
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-05-26
Journal Detail:
Title:  Developmental biology     Volume:  368     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-06-26     Completed Date:  2012-09-20     Revised Date:  2013-08-14    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  86-94     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Graduate Program in Zoology, School of Biological Sciences, The University of Texas at Austin, Austin, TX 78712, USA. dianhankuo@gmail.com
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JN565061;  JN565062
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning / genetics,  physiology*
Bone Morphogenetic Proteins / genetics,  metabolism,  physiology*
Cell Lineage / genetics
Cell Proliferation
Ectoderm / cytology,  embryology,  metabolism
Embryo, Nonmammalian / cytology,  embryology*,  metabolism
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Immunohistochemistry
In Situ Hybridization
Leeches / embryology*,  growth & development,  metabolism
Molecular Sequence Data
Signal Transduction / genetics,  physiology
Grant Support
ID/Acronym/Agency:
R01 GM074619/GM/NIGMS NIH HHS; R01 GM074619/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bone Morphogenetic Proteins
Comments/Corrections

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