| Regional differences in BMP-dependence of dorsoventral patterning in the leech Helobdella. | |
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MedLine Citation:
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PMID: 22641012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the leech Helobdella, the ectoderm exhibits a high degree of morphological homonomy between body segments, but pattern elements in lateral ectoderm arise via distinct cell lineages in the segments of the rostral and midbody regions. In each of the four rostral segments, a complete set of ventrolateral (O fate) and dorsolateral (P fate) ectodermal pattern elements arises from a single founder cell, op. In the 28 midbody and caudal segments, however, there are two initially indeterminate o/p founder cells; the more dorsal of these is induced to adopt the P fate by BMP5-8 emanating from the dorsalmost ectoderm, while the more ventral cell assumes the O fate. Previous work has suggested that the dorsoventral patterning of O and P fates differs in the rostral region, but the role of BMP signaling in those segments has not been investigated. We show here that suppression of dorsal BMP5-8 signaling (which effects a P-to-O fate change in the midbody) has no effect on the patterning of O and P fates in the rostral region. Furthermore, ectopic expression of BMP5-8 in the ventral ectoderm (which induces an O-to-P fate change in the midbody) has no effect in the rostral region. Finally, expression of a dominant-negative BMP receptor (which induces a P-to-O fate change in the midbody) fails to affect O/P patterning in the rostral region. Thus, the rostral segments appear to use some mechanism other than BMP signaling to pattern O and P cell fates along the dorsoventral axis. From a mechanistic standpoint, the OP lineage of the rostral segments and the O-P equivalence group of the midbody and caudal segments constitute distinct developmental modules that rely to differing degrees on positional cues from surrounding ectoderm in order to specify homonomous cell fates. |
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Authors:
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Dian-Han Kuo; Marty Shankland; David A Weisblat |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-05-26 |
Journal Detail:
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Title: Developmental biology Volume: 368 ISSN: 1095-564X ISO Abbreviation: Dev. Biol. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-06-26 Completed Date: 2012-09-20 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 86-94 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Graduate Program in Zoology, School of Biological Sciences, The University of Texas at Austin, Austin, TX 78712, USA. dianhankuo@gmail.com |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/JN565061; JN565062 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Patterning / genetics, physiology* Bone Morphogenetic Proteins / genetics, metabolism, physiology* Cell Lineage / genetics Cell Proliferation Ectoderm / cytology, embryology, metabolism Embryo, Nonmammalian / cytology, embryology*, metabolism Gene Expression Regulation, Developmental Gene Knockdown Techniques Immunohistochemistry In Situ Hybridization Leeches / embryology*, growth & development, metabolism Molecular Sequence Data Signal Transduction / genetics, physiology |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM074619/GM/NIGMS NIH HHS; R01 GM074619/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bone Morphogenetic Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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