Document Detail

Regional differences of the mouse brain in response to an α-linolenic acid-restricted diet: Neurotrophin content and protein kinase activity.
MedLine Citation:
PMID:  20837030     Owner:  NLM     Status:  MEDLINE    
AIMS: The purpose of this study was to determine a relatively short-term effect of feeding an α-linolenic acid (ALA, n-3)-restricted, linoleic acid (LA, n-6)-adequate diet on neurotrophin contents and protein kinase activities in brain regions of the mouse. MAIN METHODS: After feeding mice a safflower oil (SAF) diet (ALA-restricted, LA-adequate) or perilla oil (PER) diet (containing adequate amounts of ALA and LA) for 4 weeks from weaning, the fatty acid compositions of brain regions were analyzed by capillary column gas-liquid chromatography, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) contents were measured using enzyme-linked immunosorbent assay. KEY FINDINGS: The striatum and hippocampus, but not the cerebral cortex, from the SAF group, contained a smaller amount of docosahexaenoic acid (DHA, 22:6n-3) than those from the PER group. The NGF contents in these brain regions were not different between the two dietary groups. However, the striatal BDNF content of the SAF group was significantly lower than that of the PER group. Protein kinase A, protein kinase C, and p44/42 mitogen-activated protein kinase (p44/42 MAPK) activities in brain regions showed no significant difference between the two dietary groups. However, the striatal p38 MAPK activity was significantly lower in the SAF group than in the PER group. No such differences were observed in the hippocampus or the cerebral cortex. SIGNIFICANCE: A relatively short-term feeding of an α-linolenic acid-restricted, linoleic acid-adequate diet was found to lower the DHA content, BDNF content and p38 MAPK activity in the mouse striatum.
Daisuke Miyazawa; Yuko Yasui; Kazuyo Yamada; Naoki Ohara; Harumi Okuyama
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-15
Journal Detail:
Title:  Life sciences     Volume:  87     ISSN:  1879-0631     ISO Abbreviation:  Life Sci.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-11     Completed Date:  2010-10-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  490-4     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Kinjo Gakuin University College of Pharmacy, Open Research Center for Lipid Nutrition, Moriyamaku, Nagoya 463-8521, Japan.
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MeSH Terms
Brain-Derived Neurotrophic Factor / metabolism*
Cerebral Cortex / metabolism
Chromatography, Gas
Chromatography, Liquid
Corpus Striatum / metabolism
Diet, Fat-Restricted
Docosahexaenoic Acids / metabolism*
Enzyme-Linked Immunosorbent Assay
Hippocampus / metabolism
Mice, Inbred ICR
Nerve Growth Factor / metabolism
Protein Kinases / metabolism*
alpha-Linolenic Acid / administration & dosage*
p38 Mitogen-Activated Protein Kinases / metabolism*
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 25167-62-8/Docosahexaenoic Acids; 463-40-1/alpha-Linolenic Acid; 9061-61-4/Nerve Growth Factor; EC 2.7.-/Protein Kinases; EC Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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