Document Detail


Regional astrogliosis in the mouse hypothalamus in response to obesity.
MedLine Citation:
PMID:  23047490     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Obesity is associated with chronic low-grade inflammation in peripheral tissues, which contributes to the development of comorbidities such as insulin resistance and cardiovascular disease. While less extensively characterized, obesity also promotes inflammation in the central nervous system (CNS) and the consequences of this inflammation for CNS function are only beginning to be examined. In response to CNS insults such as inflammation, astrocytes undergo a process of hypertrophy and hyperplasia known as reactive astrogliosis. We used immunohistochemistry to examine the differential distribution of the astrocyte marker glial-fibrillary acidic protein (GFAP) in the brains of diet-induced or genetically obese mice compared with their respective lean controls to determine whether different nuclei of the hypothalamus showed comparable astrogliosis in response to obesity. The areas that showed the highest differential GFAP immunoreactivity between lean and obese animals include the medial preoptic, paraventricular, and dorsomedial nuclei. Comparatively, little astrogliosis was seen in the ventromedial nucleus, lateral hypothalamus, or anterior hypothalamic area. In obese animals high levels of GFAP immunoreactivity were often associated with the microvasculature. There were no differences in the differential distribution of GFAP staining between obese animals and their lean controls in the diet-induced compared with the genetic model of obesity. The exact cause(s) of the astrogliosis in obesity is not known. The finding that obesity causes a distinct pattern of elevated GFAP immunoreactivity associated with microvessels suggests that the astrogliosis may be occurring as a response to changes at the blood-brain barrier and/or in the peripheral circulation.
Authors:
Laura B Buckman; Misty M Thompson; Heidi N Moreno; Kate L J Ellacott
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  521     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-02-21     Completed Date:  2013-10-29     Revised Date:  2014-03-27    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1322-33     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / metabolism*,  pathology
Female
Gliosis / metabolism*,  pathology
Hypothalamus / metabolism*,  pathology
Mice
Mice, Inbred C57BL
Mice, Knockout
Obesity / metabolism*,  pathology
Grant Support
ID/Acronym/Agency:
DK020593/DK/NIDDK NIH HHS; DK058404/DK/NIDDK NIH HHS; DK07563/DK/NIDDK NIH HHS; P30 DK020593/DK/NIDDK NIH HHS; P30 DK058404/DK/NIDDK NIH HHS; T32 DK007563/DK/NIDDK NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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