Document Detail


Regional accretion of gelatinase B in mammary gland during gradual and acute involution of dairy animals.
MedLine Citation:
PMID:  18474138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The level of gelatinases in surrounding body fluids of actively remodelling tissue is indicative of basement membrane and extracellular matrix degradation under various physiological and pathological circumstances. To elucidate the association of gelatinase with mammary tissue remodelling during gradual or acute involution, in the first trial, goats milked twice daily (lactation) and goats receiving decreased milking frequency (involution) served to provide a total of 12 milk samples and 11 mammary secretion samples, respectively. In the second trial, 6 cows served to provide samples of dry secretion in 3 consecutive weeks immediately following milk stasis. Gelatin zymography was applied for gelatinase phenotyping and quantification on milk, plasma and the degranulation medium/lysate of milk somatic cells. Results indicated that the most prevalent gelatinase subtype switched from gelatinase A in milk to gelatinase B in involution secretion. Mammary secretion of goats during involution contained marginally higher protein level, significantly lower casein ratio and greater specific capacity of gelatinase B compared with those of milk during lactation. Specific capacities of gelatinases A and B in plasma of goats were similar during lactation and involution, while gelatinase B capacity in degranulation medium/lysates based on unit number of goat somatic cell was significantly higher during involution than during lactation. Milk stasis of cows induced a significant increase in specific capacity of gelatinase B, but not gelatinase A, of dry secretion up to the third week. Results of both trials agree that regional selective accretion of gelatinase B in milk might have played a role in mammary tissue remodelling during involution induced by either decreasing milking frequency or milk stasis. It is suggested that infiltrated polymorphonuclear neutrophils are one of the potential contributors responsible for the accumulation of gelatinase B during involution.
Authors:
Ming H Weng; Ting C Yu; Shuen E Chen; Ho C Peh; Wen B Liu; Ming T Chen; Hajemi Nagahata; Chai J Chang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of dairy research     Volume:  75     ISSN:  0022-0299     ISO Abbreviation:  J. Dairy Res.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-13     Completed Date:  2008-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  202-10     Citation Subset:  IM    
Affiliation:
Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan, ROC.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle / physiology*
Female
Goats / blood,  physiology*
Lactation / physiology*
Mammary Glands, Animal / enzymology*
Matrix Metalloproteinase 9 / analysis,  blood,  metabolism*
Milk / chemistry,  cytology
Chemical
Reg. No./Substance:
EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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