Document Detail

Regional differences in stem and transit cell proliferation and apoptosis in the terminal ileum and colon of mice after 12 Gy.
MedLine Citation:
PMID:  22196132     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The intestinal epithelium has a high rate of cell turnover, which is regulated by stem cells located near the base of crypts. We aimed to investigate stem cell-dependent characteristics of cell proliferation, apoptosis, and crypt size in terminal ileum and different regions of the colon.
METHODS AND MATERIALS: Mice were studied under steady-state conditions and after radiation-induced stem cell apoptosis. Percentage of proliferating or apoptotic cells at a particular cell position (cp) along the crypt axis was expressed as labeling or apoptotic index.
RESULTS: Under steady-state conditions: crypt size was smallest in the ascending colon. In contrast to other regions of the colon, the distribution profile of proliferating cells in ascending colon showed some similarity to that in the terminal ileum. Postirradiation: apoptotic cells were prominent at the bottom of the crypt of mid- and descending colon but in the ascending colon, they were seen with similar frequency from cp 1 to 4. During regeneration, a constant proliferative capacity was seen above Paneth cells in the terminal ileum. In the ascending (but not mid- or descending) colon, the profile of proliferating cells over the first 4 days after irradiation showed a similarity to that in the terminal ileum.
CONCLUSIONS: Profiles of proliferating epithelial cells (under steady-state conditions and postirradiation) and apoptotic cells (postirradiation) suggest similarities in the location of stem cells in the ascending colon and terminal ileum.
Ricardo M C Gândara; Yashwant R Mahida; Christopher S Potten
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-12-21
Journal Detail:
Title:  International journal of radiation oncology, biology, physics     Volume:  82     ISSN:  1879-355X     ISO Abbreviation:  Int. J. Radiat. Oncol. Biol. Phys.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-01-30     Completed Date:  2012-03-15     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  7603616     Medline TA:  Int J Radiat Oncol Biol Phys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e521-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Apoptosis / radiation effects*
Cell Movement / physiology,  radiation effects
Cell Proliferation / radiation effects*
Colon / cytology,  physiology,  radiation effects*
Ileum / cytology,  physiology,  radiation effects*
Paneth Cells / physiology
Radiation Dosage
Regeneration / physiology
Stem Cells / physiology,  radiation effects*
Grant Support
G0100577//Medical Research Council; G0500067//Medical Research Council; //Medical Research Council

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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