Document Detail


Regional cell shape changes control form and function of Kupffer's vesicle in the zebrafish embryo.
MedLine Citation:
PMID:  22841644     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cilia-generated fluid flow in an 'organ of asymmetry' is critical for establishing the left-right body axis in several vertebrate embryos. However, the cell biology underlying how motile cilia produce coordinated flow and asymmetric signals is not well defined. In the zebrafish organ of asymmetry-called Kupffer's vesicle (KV)-ciliated cells are asymmetrically positioned along the anterior-posterior axis such that more cilia are placed in the anterior region. We previously demonstrated that Rho kinase 2b (Rock2b) is required for anteroposterior asymmetry and fluid flow in KV, but it remained unclear how the distribution of ciliated cells becomes asymmetric during KV development. Here, we identify a morphogenetic process we refer to as 'KV remodeling' that transforms initial symmetry in KV architecture into anteroposterior asymmetry. Live imaging of KV cells revealed region-specific cell shape changes that mediate tight packing of ciliated cells into the anterior pole. Mathematical modeling indicated that different interfacial tensions in anterior and posterior KV cells are involved in KV remodeling. Interfering with non-muscle myosin II (referred to as Myosin II) activity, which modulates cellular interfacial tensions and is regulated by Rock proteins, disrupted KV cell shape changes and the anteroposterior distribution of KV cilia. Similar defects were observed in Rock2b depleted embryos. Furthermore, inhibiting Myosin II at specific stages of KV development perturbed asymmetric flow and left-right asymmetry. These results indicate that regional cell shape changes control the development of anteroposterior asymmetry in KV, which is necessary to generate coordinated asymmetric fluid flow and left-right patterning of the embryo.
Authors:
Guangliang Wang; M Lisa Manning; Jeffrey D Amack
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-26
Journal Detail:
Title:  Developmental biology     Volume:  370     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-03     Completed Date:  2012-11-08     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  52-62     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Department of Cell and Developmental Biology, State University of New York, Upstate Medical University, Syracuse, NY 13210, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning / physiology*
Cell Shape / physiology*
Cilia / physiology*
Fluorescence
Heterocyclic Compounds with 4 or More Rings
Immunohistochemistry
In Situ Hybridization
Models, Biological*
Morphogenesis / physiology*
Morpholinos
Myosin Type II / metabolism
Zebrafish / embryology*
rho-Associated Kinases / metabolism
Grant Support
ID/Acronym/Agency:
R01 HL095690/HL/NHLBI NIH HHS; R01HL095690/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Heterocyclic Compounds with 4 or More Rings; 0/Morpholinos; 0/blebbistatin; EC 2.7.11.1/rho-Associated Kinases; EC 3.6.1.-/Myosin Type II
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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