Document Detail

Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise.
MedLine Citation:
PMID:  19713429     Owner:  NLM     Status:  MEDLINE    
Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.
U R Mikkelsen; H Langberg; I C Helmark; D Skovgaard; L L Andersen; M Kjaer; A L Mackey
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-08-27
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  107     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-05     Completed Date:  2010-01-13     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1600-11     Citation Subset:  IM    
Institute of Sports Medicine, Bispebjerg Hospital, Bispebjerg Bakke 23, Copenhagen DK-2400, Denmark.
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MeSH Terms
Anti-Inflammatory Agents, Non-Steroidal / administration & dosage*
Cell Proliferation / drug effects
Cytokines / metabolism*
Indomethacin / administration & dosage*
Infusions, Parenteral
Injections, Intramuscular
Muscle, Skeletal / cytology*,  drug effects,  physiology*
Physical Endurance / drug effects,  physiology*
Physical Exertion / drug effects,  physiology
Satellite Cells, Skeletal Muscle / cytology,  drug effects,  physiology*
Young Adult
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Cytokines; 53-86-1/Indomethacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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