Document Detail


Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise.
MedLine Citation:
PMID:  19713429     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.
Authors:
U R Mikkelsen; H Langberg; I C Helmark; D Skovgaard; L L Andersen; M Kjaer; A L Mackey
Related Documents :
11758569 - Leg venous compliance in orthostatic intolerance before and after 14-day head-down bed ...
12475469 - Isometric arm counter-pressure maneuvers to abort impending vasovagal syncope.
7067509 - Physical training fails to improve ventilatory muscle endurance in patients with chroni...
15121799 - Maximal muscular vascular conductances during whole body upright exercise in humans.
12582149 - Skiing across the greenland icecap: divergent effects on limb muscle adaptations and su...
20204819 - Decreased muscle oxygenation and increased arterial blood flow in the non-exercising li...
20363849 - Bronchial epithelial damage after a half-marathon in nonasthmatic amateur runners.
9032509 - The effect of dipyridamole and theophylline on hypercapnic ventilatory responses: the r...
14627299 - Microbial and sensory quality of marinated and irradiated chicken.
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-08-27
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  107     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-05     Completed Date:  2010-01-13     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1600-11     Citation Subset:  IM    
Affiliation:
Institute of Sports Medicine, Bispebjerg Hospital, Bispebjerg Bakke 23, Copenhagen DK-2400, Denmark. ullaramer@hotmail.com
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Anti-Inflammatory Agents, Non-Steroidal / administration & dosage*
Cell Proliferation / drug effects
Cytokines / metabolism*
Humans
Indomethacin / administration & dosage*
Infusions, Parenteral
Injections, Intramuscular
Male
Muscle, Skeletal / cytology*,  drug effects,  physiology*
Physical Endurance / drug effects,  physiology*
Physical Exertion / drug effects,  physiology
Satellite Cells, Skeletal Muscle / cytology,  drug effects,  physiology*
Young Adult
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Cytokines; 53-86-1/Indomethacin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Surround inhibition depends on the force exerted and is abnormal in focal hand dystonia.
Next Document:  Evidence against a 40 degrees C core temperature threshold for fatigue in humans.