Document Detail


Region [corrected] of slowed conduction acts as core for spiral wave reentry in cardiac cell monolayers.
MedLine Citation:
PMID:  17965287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pathophysiological heterogeneity in cardiac tissue is related to the occurrence of arrhythmias. Of importance are regions of slowed conduction, which have been implicated in the formation of conduction block and reentry. Experimentally, it has been a challenge to produce local heterogeneity in a manner that is both reversible and well controlled. Consequently, we developed a dual-zone superfusion chamber that can dynamically create a small (5 mm) central island of heterogeneity in cultured cardiac cell monolayers. Three different conditions were studied to explore the effect of regionally slowed conduction on wave propagation and reentry: depolarization by elevated extracellular potassium, sodium channel inhibition with lidocaine, and cell-cell decoupling with palmitoleic acid. Using optical mapping of transmembrane voltage, we found that the central region of slowed conduction always served as the core region around which a spiral wave formed and then revolved following a period of rapid pacing. Because of the localized slowing in the core region, we observed experimentally for the first time an S shape of the spiral wave front near its tip. These results indicate that a small region of slowed conduction can play a crucial role in the formation, anchoring, and modulation of reentrant spiral waves.
Authors:
Joyce W Lin; Libet Garber; Yue Rosa Qi; Marvin G Chang; Joshua Cysyk; Leslie Tung
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-10-26
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  294     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-14     Completed Date:  2008-02-28     Revised Date:  2008-04-30    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H58-65     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, The Johns Hopkins University, 720 Rutland Avenue, Baltimore, MD 21205, USA.
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MeSH Terms
Descriptor/Qualifier:
Action Potentials* / drug effects
Animals
Animals, Newborn
Anti-Arrhythmia Agents / pharmacology
Arrhythmias, Cardiac / metabolism*,  physiopathology
Cardiac Pacing, Artificial
Cell Culture Techniques / instrumentation*
Cells, Cultured
Equipment Design
Fatty Acids, Monounsaturated / pharmacology
Heart Ventricles / cytology,  metabolism
Lidocaine / pharmacology
Myocytes, Cardiac / drug effects,  metabolism*
Potassium / metabolism
Rats
Rats, Sprague-Dawley
Signal Processing, Computer-Assisted
Time Factors
Grant Support
ID/Acronym/Agency:
R01 HL 66239/HL/NHLBI NIH HHS; R21 EB 006171/EB/NIBIB NIH HHS; R21 RR 017073/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Arrhythmia Agents; 0/Fatty Acids, Monounsaturated; 137-58-6/Lidocaine; 2091-29-4/palmitoleic acid; 7440-09-7/Potassium
Comments/Corrections
Erratum In:
Am J Physiol Heart Circ Physiol. 2008 Mar;294(3):H1501

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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