Document Detail


Regio- and enantioselective oxidation of thiaoleic acids by an algal delta 12-desaturase.
MedLine Citation:
PMID:  10570826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a non aerated incubation medium, the oxidation of the tested thiaoleic acids by C. vulgaris was regioselective. Moreover the non oxidised 14-thiaoleic acid had no influence on the endogenous fatty acid pattern whereas the 13-thiaoleic acid, readily oxidised by the microalgae, caused a significant 18:1 accumulation in phospholipids. Examination of the 13-thiaoleic acid metabolism revealed that up to 15% of the substrate was specifically oxidised in the phospholipids before being transferred into the neutral lipids class of C. vulgaris. Confirmation of the monooxygenase-like delta 12-desaturase intervention in this biotransformation was obtained by the stereochemical analysis of the optically active sulfoxide isolated from the endoplasmic phospholipids.
Authors:
L Fauconnot; C Nugier-Chauvin; N Noiret; S Poulain; H Patin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytochemistry     Volume:  52     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-12-20     Completed Date:  1999-12-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  567-73     Citation Subset:  IM    
Affiliation:
Laboratoire de Chimie Organique et des Substances Naturelles, ESA CNRS 6052, Ecole Nationale Supérieure de Chimie de Rennes, France.
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MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / metabolism
Biotransformation
Cells, Cultured
Chlorella / enzymology*,  metabolism
Lipid Metabolism
Oleic Acids / metabolism*
Oxidation-Reduction
Phospholipids / metabolism
Stearoyl-CoA Desaturase / metabolism*
Stereoisomerism
Substrate Specificity
Sulfoxides / metabolism
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Oleic Acids; 0/Phospholipids; 0/Sulfoxides; EC 1.14.19.1/Stearoyl-CoA Desaturase

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