Document Detail

Regeneration of transgenic Lycium barbarum L.
MedLine Citation:
PMID:  1668526     Owner:  NLM     Status:  MEDLINE    
A simple and effective system for the transformation and regeneration of Lycium barbarum L. has been developed. Young stem segments from Lycium barbarum L. were infected by Agrobacterium tumefaciens harboring a vector containing neomycin phosphotransferase II (npt-II) gene derived from non-oncogenic Ti plasmid. Calli originating from young stem segments on selective induction medium could differentiate into buds on selective differentiation medium rapidly and finally developed into whole plants. NPT-II enzyme activity assay and DNA hybridization indicated that the foreign gene had been integrated into the genome of Lycium barbarum L. and could be expressed in plants.
H Wang; F Huang; A Li; Q Shao; D Niu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese journal of biotechnology     Volume:  7     ISSN:  1042-749X     ISO Abbreviation:  Chin. J. Biotechnol.     Publication Date:  1991  
Date Detail:
Created Date:  1992-09-25     Completed Date:  1992-09-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9100855     Medline TA:  Chin J Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  185-9     Citation Subset:  IM    
Institute of Genetics, Chinese Academy of Sciences, Beijing.
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MeSH Terms
Kanamycin / pharmacology
Kanamycin Kinase
Kanamycin Resistance / genetics
Phosphotransferases / genetics*
Plants, Genetically Modified / anatomy & histology,  enzymology,  genetics,  growth & development*
Plants, Medicinal / genetics,  growth & development
Transformation, Genetic*
Reg. No./Substance:
59-01-8/Kanamycin; EC 2.7.-/Phosphotransferases; EC Kinase

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