Document Detail

Regeneration of pea (Pisum sativum L.) by a cyclic organogenic system.
MedLine Citation:
PMID:  15372196     Owner:  NLM     Status:  MEDLINE    
In a five-step procedure, plants were regenerated from meristematic tissue initiated from nodal tissue in four pea cultivars ('Espace', 'Classic', 'Solara', and 'Puget'). In step 1, stem tissue with one node (1-cm size) was subcultured on medium containing thidiazuron. As a result multiple shoots were produced, appearing normal or swollen at their bases. The multiple shoots were subcultured in the same medium, resulting in the formation of a green hyperhydric tissue in the swollen bases of the multiple shoots, which is fully covered with small buds [bud-containing tissue (BCT)]. In step 2, BCT fragments were isolated and subcultured in the same medium and, as a result, they were able to reproduce themselves in a cyclic fashion. In step 3, subculture of BCT on medium supplemented with a combination of gibberelic acid, 6-benzyladenine and alpha-naphthalene acetic acid (NAA), resulted in the formation of shoots, which were rooted in step 4 on medium supplemented with 0.5 mg/l NAA, indole-3-acetic acid (IAA) or indole-3-butyric acid. In step 5, in vitro plants were transferred to the greenhouse for acclimatisation and further development. The four varieties tested were all able to produce meristematic tissue, suggesting that its production is genotype independent.
Emmanouil N Tzitzikas; Marjan Bergervoet; Krit Raemakers; Jean-Paul Vincken; Andre van Lammeren; Richard G F Visser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-09-15
Journal Detail:
Title:  Plant cell reports     Volume:  23     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-10-17     Completed Date:  2005-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  453-60     Citation Subset:  IM    
Laboratory of Plant Breeding, The Graduate School Experimental Plant Sciences, Wageningen University, Binnenhaven 5, 6709 PD, Wageningen, The Netherlands.
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MeSH Terms
Acclimatization / physiology
Agriculture / methods
Culture Media / chemistry,  pharmacology
Meristem / drug effects,  growth & development,  metabolism
Peas / drug effects,  growth & development*,  metabolism
Plant Growth Regulators / pharmacology
Plant Shoots / drug effects,  growth & development,  metabolism
Regeneration / drug effects,  physiology*
Reg. No./Substance:
0/Culture Media; 0/Plant Growth Regulators

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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