Document Detail


Regeneration in zebrafish lateral line neuromasts: expression of the neural progenitor cell marker sox2 and proliferation-dependent and-independent mechanisms of hair cell renewal.
MedLine Citation:
PMID:  17443814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mechanosensory hair cells are essential for audition in vertebrates, and in many species, have the capacity for regeneration when damaged. Regeneration is robust in the fish lateral line system as new hair cells can reappear after damage induced by waterborne aminoglycoside antibiotics, platinum-based drugs, and heavy metals. Here, we characterize the loss and reappearance of lateral line hair cells induced in zebrafish larvae treated with copper sulfate using diverse molecular markers. Transgenic fish that express green fluorescent protein in different cell types in the lateral line system have allowed us to follow the regeneration of hair cells after different damage protocols. We show that conditions that damage only differentiated hair cells lead to reappearance of new hair cells within 24 h from nondividing precursors, whereas harsher conditions are followed by a longer recovery period that is accompanied by extensive cell division. In order to characterize the cell population that gives rise to new hair cells, we describe the expression of a neural stem cell marker in neuromasts. The zebrafish sox2 gene is strongly expressed in neuromast progenitor cells, including those of the migrating lateral line primordium, the accessory cells that underlie the hair cells in neuromasts, and in interneuromastic cells that give rise to new neuromasts. Moreover, we find that most of the cells that proliferate within the neuromast during regeneration express this marker. Thus, our results describe the dynamics of hair cell regeneration in zebrafish and suggest the existence of at least two mechanisms for recovery of these cells in neuromasts.
Authors:
Pedro P Hernández; Francisco A Olivari; Andrés F Sarrazin; Pablo C Sandoval; Miguel L Allende
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental neurobiology     Volume:  67     ISSN:  1932-8451     ISO Abbreviation:  Dev Neurobiol     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-19     Completed Date:  2007-06-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101300215     Medline TA:  Dev Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  637-54     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2007 Wiley Periodicals, Inc.
Affiliation:
Millennium Nucleus in Developmental Biology, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Antimetabolites / diagnostic use
Bromodeoxyuridine / diagnostic use
Cell Proliferation / drug effects
Copper / toxicity
DNA-Binding Proteins / biosynthesis*,  genetics
Green Fluorescent Proteins / biosynthesis,  genetics
HMGB Proteins / biosynthesis*,  genetics
Hair Cells, Auditory / drug effects,  physiology*
Image Processing, Computer-Assisted
Immunohistochemistry
In Situ Hybridization
Lateral Line System / physiology*
Mitosis / physiology
Nerve Regeneration / physiology
Neurons / physiology*
SOXB1 Transcription Factors
Stem Cells / drug effects,  physiology*
Transcription Factors / biosynthesis*,  genetics
Zebrafish
Chemical
Reg. No./Substance:
0/Antimetabolites; 0/DNA-Binding Proteins; 0/HMGB Proteins; 0/SOXB1 Transcription Factors; 0/Transcription Factors; 147336-22-9/Green Fluorescent Proteins; 59-14-3/Bromodeoxyuridine; 7440-50-8/Copper

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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