| Regeneration of cation-transport capacity in HeLa cell membranes after specific blockade by ouabain. | |
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MedLine Citation:
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PMID: 4506784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cardiac glycoside, ouabain, inhibits alkali-cation transport in HeLa cells. It binds to 0.75 x 10(6) sites per cell, and the half-time for its dissociation is 16 hr. After partial blockade by ouabain, the cell generates new ouabain-binding sites, with total restoration of transport in 10% of a cell cycle( approximately 3 hr). This recovery requires protein synthesis and appears to be a response to altered cell-electrolyte content, since growth of cells in media with low K(+) concentration enhances the titer of the transport enzyme in a fashion similar to the effect of ouabain. Totally blocked cells do not recover. |
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Authors:
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G L Vaughan; J S Cook |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 69 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1972 Sep |
Date Detail:
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Created Date: 1972-11-10 Completed Date: 1972-11-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2627-31 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Biological Transport / drug effects Cell Line Cells, Cultured Cycloheximide / pharmacology Hela Cells / drug effects, growth & development, metabolism* Kinetics Leucine / metabolism Mice Neoplasm Proteins / biosynthesis Ouabain / metabolism, pharmacology* Potassium / pharmacology Radioisotopes Rubidium / metabolism* Tritium |
| Chemical | |
Reg. No./Substance:
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0/Neoplasm Proteins; 0/Radioisotopes; 10028-17-8/Tritium; 61-90-5/Leucine; 630-60-4/Ouabain; 66-81-9/Cycloheximide; 7440-09-7/Potassium; 7440-17-7/Rubidium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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