Document Detail

Regeneration of cation-transport capacity in HeLa cell membranes after specific blockade by ouabain.
MedLine Citation:
PMID:  4506784     Owner:  NLM     Status:  MEDLINE    
The cardiac glycoside, ouabain, inhibits alkali-cation transport in HeLa cells. It binds to 0.75 x 10(6) sites per cell, and the half-time for its dissociation is 16 hr. After partial blockade by ouabain, the cell generates new ouabain-binding sites, with total restoration of transport in 10% of a cell cycle( approximately 3 hr). This recovery requires protein synthesis and appears to be a response to altered cell-electrolyte content, since growth of cells in media with low K(+) concentration enhances the titer of the transport enzyme in a fashion similar to the effect of ouabain. Totally blocked cells do not recover.
G L Vaughan; J S Cook
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Sep 
Date Detail:
Created Date:  1972-11-10     Completed Date:  1972-11-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2627-31     Citation Subset:  IM    
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MeSH Terms
Binding Sites
Biological Transport / drug effects
Cell Line
Cells, Cultured
Cycloheximide / pharmacology
Hela Cells / drug effects,  growth & development,  metabolism*
Leucine / metabolism
Neoplasm Proteins / biosynthesis
Ouabain / metabolism,  pharmacology*
Potassium / pharmacology
Rubidium / metabolism*
Reg. No./Substance:
0/Neoplasm Proteins; 0/Radioisotopes; 10028-17-8/Tritium; 61-90-5/Leucine; 630-60-4/Ouabain; 66-81-9/Cycloheximide; 7440-09-7/Potassium; 7440-17-7/Rubidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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