Document Detail


Regeneration of acinar cells following ligation of rat submandibular gland retraces the embryonic-perinatal pathway of cytodifferentiation.
MedLine Citation:
PMID:  20056310     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rat submandibular gland can regenerate following ligation-induced atrophy, eventually recovering its normal morphology and function. Previous studies have suggested that the regeneration process implies both self-proliferation of existing acini and formation of new acinar cells. One hypothesis is that new acinar cells may differentiate from the ductal cells in a similar fashion to the process of cytodifferentiation occurring during submandibular glandular development. In this study atrophy was induced, under recovery anaesthesia, by applying a metal clip on the main duct of the submandibular gland without including the chorda lingual nerve. After 2 weeks the duct was deligated for 3, 5 or 7 days or 8 weeks and the glands collected. Tissue was prepared for immunohistochemistry, biochemical analysis and RNA extraction. The histology of the regenerated glands shows several normal-looking acini, which have regained their glycoprotein content (AB/PAS positive), data also confirmed by biochemical analysis (SDS-PAGE/PAS). Regenerating tissue was characterized by the presence of embryonic-like branched structures ending with AB/PAS positive acinar cells. The proteins SMG-B and PSP are normally expressed in acinar cell precursors during development but only by intercalated ductal cells in the adult stage. In the adult regenerating gland mRNA levels of both SMG-B and PSP were found to be up-regulated compared to ligated glands and SMG-B expression localized to acinar cells whilst the ductal cells were negative. This study of rat submandibular gland regeneration suggests new acinar cells have differentiated from ducts and express markers of acinar cell precursors in a similar manner to the cytodifferentiation process occurring during glandular development.
Authors:
Emanuele Cotroneo; Gordon B Proctor; Guy H Carpenter
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-06
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  79     ISSN:  1432-0436     ISO Abbreviation:  Differentiation     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-03-11     Completed Date:  2010-06-22     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  England    
Other Details:
Languages:  eng     Pagination:  120-30     Citation Subset:  IM    
Copyright Information:
2009 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.
Affiliation:
King's College London Dental Institute, London,UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation*
Cell Proliferation
Cells, Cultured
Embryo, Mammalian / metabolism*
Ligation
Rats
Rats, Wistar
Regeneration
Salivary Ducts / cytology,  metabolism
Submandibular Gland / cytology*,  physiology*
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Comments/Corrections

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