| Regeneration of Three Layers Vascular Wall by using BMP2-Treated MSC Involving HIF-1α and Id1 Expressions Through JAK/STAT Pathways. | |
| | |
MedLine Citation:
|
PMID: 21472453 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Engineering living, multilayered blood vessels to form in vivo arteries is a promising alternative to peripheral artery bypass using acellular grafts restricted by thrombosis and occlusion at long term. Bone Morphogenetic Protein 2 (BMP2) is a growth factor determining in the early vascular embryonic development. The aim of the present study was evaluate the collaborative effect of recombinant human-BMP2 and Bone marrow-Mesenchymal stem cells (BM-MSCs) seeded on vascular patch to regenerate a vascular arterial wall in a rat model. BM-MSCs expressing green fluorescent protein (GFP) seeded on vascular patch were cultured in presence of recombinant human-BMP2 [100 ng/mL] during 1 week before their implantation on the abdominal aorta of Wistar rats. We observed after 2 weeks under physiological arterial flow a regeneration of a three layers adult-like arterial wall with a middle layer expressing smooth muscle proteins and a border layer expressing endothelial marker. In vitro study, using Matrigel assay and co-culture of BM-MSCs with endothelial cells demonstrated that rh-BMP2 promoted tube-like formation even at long term (90 days) allowing the organization of thick rails. We demonstrated using inhibitors and siRNAs that rh-BMP2 enhanced the expression of HIF-1α and Id1 through, at least in part, the stimulation of JAK2/STAT3/STAT5 signaling pathways. Rh-BMP2 by mimicking embryological conditions allowed vascular BM-MSCs differentiation. |
| | |
Authors:
|
Karim Belmokhtar; Thierry Bourguignon; Morel E Worou; Georges Khamis; Pierre Bonnet; Jorge Domenech; Véronique Eder |
Related Documents
:
|
19876833 - Critical growth factors and signalling pathways controlling human trophoblast invasion. 20501323 - Anti-chondrosarcoma effects of pedf mediated via molecules important to apoptosis, cell... 12437673 - The expression of prion protein by endothelial cells: a source of the plasma form of pr... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-4-7 |
Journal Detail:
|
Title: Stem cell reviews Volume: - ISSN: 1558-6804 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-4-7 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101255952 Medline TA: Stem Cell Rev Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Faculty of Medicine, University François Rabelais, Labpart EA 3852, IFR 135, Tours, France, karimbelmokhtar@hotmail.fr. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro inhibitory effect of trichostatin A on canine grade 3 mast cell tumor.
Next Document: Mercury Speciation in Fish Tissues from a Mediterranean River Basin: The Tagus River (Central Spain)...