Document Detail

Refrigerator storage of expressed human milk in the neonatal intensive care unit.
MedLine Citation:
PMID:  19783003     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To provide recommendations for refrigerator storage of human milk, the overall integrity (bacterial growth, cell counts, and component concentrations) of milk was examined during 96 hours of storage at 4 degrees C. STUDY DESIGN: Fresh milk samples (n = 36) were divided and stored at 4 degrees C for 0, 24, 48, 72, and 96 hours. At each time, pH, white cell count, and osmolality were measured and additional samples were stored at -80 degrees C until analyzed for bacteria and concentrations of lactoferrin, secretory (s)IgA, fat, fatty acids, and protein. RESULTS: There were no significant changes for osmolality, total and Gram-negative bacterial colony counts or concentrations of sIgA, lactoferrin, and fat. Gram-positive colony counts (2.9 to 1.6 x 10(5) colony-forming units per mL), pH (7.21 to 6.68), white blood cell counts (2.31 to 1.85 x 10(6) cells per mL), and total protein (17.5 to 16.7 g/L) declined, and free fatty acid concentrations increased (0.35 to 1.28 g/L) as storage duration increased, P < .001. CONCLUSIONS: Changes were minimal and the overall integrity of milk during refrigerator storage was preserved. Fresh mother's milk may be stored at refrigerator temperature for as long as 96 hours.
Meredith Slutzah; Champa N Codipilly; Debra Potak; Richard M Clark; Richard J Schanler
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  The Journal of pediatrics     Volume:  156     ISSN:  1097-6833     ISO Abbreviation:  J. Pediatr.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-02-17     Revised Date:  2010-08-16    
Medline Journal Info:
Nlm Unique ID:  0375410     Medline TA:  J Pediatr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  26-8     Citation Subset:  AIM; IM    
Division of Neonatal-Perinatal Medicine, Schneider Children's Hospital, New Hyde Park, NY, USA.
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MeSH Terms
Colony Count, Microbial
Fatty Acids, Nonesterified / analysis
Hydrogen-Ion Concentration
Intensive Care Units, Neonatal*
Milk, Human / chemistry
Osmolar Concentration
Time Factors
Reg. No./Substance:
0/Fatty Acids, Nonesterified
Comment In:
J Hum Lact. 2010 Aug;26(3):233-4   [PMID:  20689101 ]

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